|
| Status |
Public on May 23, 2021 |
| Title |
Young and Old Pre- and Post-Transplantation ATAC Seq |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ATAC-sequencing of paired Y and O mouse HSCs pre and 3mo post lethal irradiation and HSC transplantation
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| |
|
| Overall design |
For the ATAC-seq analyses of Y and O HSCs pre- and post-transplantation, we utilized a paired approach. For each replicate, pools of 3 to 4 Y B6 mice and 1 O B6 mouse were used to isolated 10,000 pre-transplantation HSCs for ATAC-seq sample preparation and to transplant 1,000 HSCs into 5 lethally-irradiated Y BJ recipient mice alongside 300,000 Sca-1 depleted BJ helper BM cells. Post-transplantation samples were collected 3 months after transplantation from the pooled BM of the 5 recipient mice to obtain 10,000 post-transplantation HSCs for ATAC-seq sample preparation.
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| |
|
| Contributor(s) |
Dellorusso P, Passegué E |
| Citation(s) |
34032859 |
| |
| Submission date |
Jun 02, 2020 |
| Last update date |
Jun 29, 2022 |
| Contact name |
Paul Vincent Dellorusso |
| E-mail(s) |
[email protected]
|
| Phone |
5166552491
|
| Organization name |
Columbia University Irving Medical Center
|
| Department |
Genetics and Development
|
| Lab |
Passegue Lab
|
| Street address |
701 West 168th Street, 724 Hammer Health Sciences Center
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA636794 |
| SRA |
SRP265644 |
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