GEO DataSets

(Submitter supplied) ATAC-sequencing of paired Y and O mouse HSCs pre and 3mo post lethal irradiation and HSC transplantation

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21103

16 Samples

Download data: NARROWPEAK

(Submitter supplied) RNA sequencing of hematopoietic stem cells isolated from young (8-12 week) and old (24-27 month) BL6 mice from experimental groups that are intended to describe the transcriptomic consequences of parabiosis on the function of old hematopoietic stem cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

30 Samples

Download data: CSV

(Submitter supplied) Aging of hematopoietic stem cells (HSCs) is associated with the decline of their regenerative capacity, and multi-lineage differentiation potential, contributing to development of blood disorders. The bone marrow microenvironment was recently suggested to influence HSC aging, however the underlying mechanisms remain largely unknown. Here, we show that HSC aging critically depends on bone marrow innervation by the sympathetic nervous system (SNS), as premature loss of SNS nerves or adrenoreceptor b3 (ADRb3) signaling in the microenvironment accelerated the appearance of HSC aging phenotypes reminiscent of physiological aging. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

9 Samples

Download data: XLSX

(Submitter supplied) We sequenced mRNA from young or old MkP populations identified in the mouse in order to determine novel molecular regulators of their unique functional properties.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

7 Samples

Download data: TXT

(Submitter supplied) Comprehensive analysis of gene expression in hematopoietic stem and progenitor cells from young and old mice.

Organism:

Mus musculus

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL1261

71 Samples

Download data: CEL, TXT

(Submitter supplied) In this study, we investigated signaling pathways in Skeletal muscle precursors that are altered with aging and age-related deficits in muscle regenerative potential. We performed fluorescence activated cell sorting (FACS) to obtain highly purified skeletal muscle satellite cells from young, middle-aged and old mice. Parabiosis experiments indicate that impaired regeneration in aged mice is reversible by exposure to a young circulation, suggesting that young blood contains humoral "rejuvenating" factors that can restore regenerative function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4892

Platform:

GPL1261

14 Samples

Download data: CEL

Analysis of FACS-sorted CD45-Ter119-Sca-1-CD29+Cxcr4+ skeletal muscle precursor (SMP) cells from young, middle-aged, and old C57BL/6 males. Results provide insight into the molecular mechanisms underlying age-related skeletal muscle and stem cell dysfunction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age sets

Platform:

GPL1261

Series:

GSE50821

14 Samples

Download data: CEL

(Submitter supplied) Cell cycle quiescence is a critical feature contributing to haematopoietic stem cell (HSC) maintenance. Although various candidate stromal cells have been identified as potential HSC niches, the spatial localization of quiescent HSC in the bone marrow (BM) remains unclear. Here, using a novel approach that combines whole-mount confocal immunofluorescence imaging technique and computational modelling to analyse significant tridimensional associations among vascular structures, stromal cells and HSCs, we show that quiescent HSCs associate specifically with small arterioles that are preferentially found in endosteal BM. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: FPKM_TRACKING

(Submitter supplied) As part of a study of the role of the aryl hydrocarbon receptor (Ahr) in maintenance and senescence of hematopoietic stem cells (HSC), global gene expression profiling was done with HSC isolated from Ahr-knockout and wild-type mice. HSC from young-adult (8 wk old) AhR-KO mice had changes in expression of many genes related to HSC maintenance, consistent with the phenotype observed in aging Ahr-KO mice: decreased survival rate, splenomegaly, increased circulating white blood cells, hematopoietic cell accumulation in tissues, anemia, increased numbers of stem/progenitor and lineage-committed cells in bone marrow, decreased erythroid progenitor cells in bone marrow, and decreased self-renewal capacity of HSC.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

7 Samples

Download data: CEL

(Submitter supplied) Aging of hematopoietic stem cells (HSCs) leads to several functional changes, including alterations affecting self-renewal and differentiation. While it is well established that many of the age-induced changes are intrinsic to HSCs, less is known about the stability of this state. Here, we entertained the hypothesis that HSC aging is driven by the acquisition of permanent genetic mutations. To examine this issue at a functional level in vivo, we applied induced pluripotent stem (iPS) cell reprogramming of aged hematopoietic progenitors and allowed the resulting aged-derived iPS cells to reform hematopoiesis via blastocyst complementation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

16 Samples

Download data: CEL

(Submitter supplied) Somatic stem cells mediate tissue maintenance for the lifetime of an organism. Despite the well-established longevity that is a prerequisite for such function, accumulating data argue for compromised stem cell function with age. Identifying the mechanisms underlying age-dependent stem cell dysfunction is therefore key to understand the aging process. Using a model that carries a proofreading defective mitochondrial DNA polymerase, we demonstrate hematopoietic defects reminiscent of premature HSC aging including anemia, lymphopenia and myeloid lineage skewing. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3976

Platform:

GPL1261

16 Samples

Download data: CEL

Analysis of hematopoietic stem cells (HSCs) from mid-aged mutator females that express a proofreading-defective form of the mitochondrial DNA polymerase gamma (POLG). Results provide insight into the molecular mechanisms underlying multiple aspects of aging accelerated in the mutator model.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age, 2 cell type, 5 genotype/variation sets

Platform:

GPL1261

Series:

GSE27686

16 Samples

Download data: CEL

(Submitter supplied) Activation of mostly quiescent hematopoietic stem cells (HSC) is a prerequisite for life-long blood production. This process requires major molecular adaptations to meet the regulatory and metabolic requirements for cell division. The mechanisms governing cellular reprograming upon stem cell activation and their subsequent return to quiescence are still not fully characterized. Here, we describe a central role for a selective type of autophagy (CMA) in sustaining adult HSC function both through to stem cell protein quality control and to timely stimulation of linoleic fatty acid metabolism upon HSC activation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

4 Samples

Download data: CEL

(Submitter supplied) Single-cell RNA sequencing data of Mus Musculus heterochronic parabionts

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

1 Sample

Download data: H5AD

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

996 Samples

Download data: CSV, H5AD, LOOM, TAR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL24247

78 Samples

Download data

(Submitter supplied) To gain insight into the mechanisms by which exercise affects the neural stem cell compartment, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of cells from the subventricular zone of the brain of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: RDATA

(Submitter supplied) To gain insight into the mechanisms by which exercise affects the muscle stem cell compartment, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of mononucleated cells from hindlimb muscles of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: RDATA

(Submitter supplied) To gain insight into the mechanisms by which exercise affects hematopoietic stem cells, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of hematopoietic progenitors in the bone marrow of these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: RDS

(Submitter supplied) To gain insight into the mechanisms by which exercise affects circulating immune cells, we subjected young and old mice to aerobic exercise and performed single cell transcriptome analysis of circulating immune cells from these animals.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: RDS