Similar studies for GEO DataSets (Select 200117603) - GEO DataSets

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Items: 20

Select item 2001176031.

DROSHA complementation experiments using small RNA sequencing

(Submitter supplied) DROSHA complementation experiments were performed to check whether dsRBD of DROSHA recognizes the lower stem of pri-miRNAs and affects miRNA biogenesis.

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL16791
17 Samples

Download data: TXT

Series

Accession:: GSE117603

ID:: 200117603

PubMed Similar studies SRA Run Selector

Select item 2001176002.

High-throughput in vitro DROSHA processing on 38,880 pri-miRNA variants

(Submitter supplied) To investigate the precise processing mechanism of pri-miRNA, we prepared 38,880 pri-miRNA variants which contain unique barcode sequences (Fang and Bartel, Mol Cell, 60: 131) and performed high-throughput processing experiments.

Organism:: synthetic construct

Type:: Other

Platform:: GPL19604
3 Samples

Download data: TXT

Series

Accession:: GSE117600

ID:: 200117600

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Select item 2000936533.

Genome-wide mapping of DROSHA cleavage sites on primary microRNAs and novel substrates

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Other

Platform:: GPL16791
13 Samples

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Series

Accession:: GSE93653

ID:: 200093653

PubMed Similar studies

Select item 2000936514.

Genome-wide mapping of DROSHA cleavage sites on primary microRNAs and novel substrates [fCLIP-seq]

(Submitter supplied) MicroRNA (miRNA) maturation is initiated by DROSHA, a double-stranded RNA (dsRNA)-specific RNase III enzyme. By cleaving primary miRNAs (pri-miRNAs) at specific positions, DROSHA serves as a main determinant of miRNA sequences and a highly selective gate-keeper for the canonical miRNA pathway. However, the sites of DROSHA-mediated processing have not been annotated on a genomic scale, and it remains unclear to what extent DROSHA functions outside the miRNA pathway. more...

Organism:: Homo sapiens

Type:: Other

Platform:: GPL16791
4 Samples

Download data: TSV

Series

Accession:: GSE93651

ID:: 200093651

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Select item 2000936505.

Genome-wide mapping of DROSHA cleavage sites on primary microRNAs and novel substrates [RNA-seq]

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
9 Samples

Download data: TSV

Series

Accession:: GSE93650

ID:: 200093650

Select item 2001580606.

The bulges control in pri-miRNA processing in the position and strand-dependent manner

(Submitter supplied) The microRNA (miRNA) biogenesis is responsible for the production of miRNAs that play critical roles in gene expression and numerous human diseases. The adequate biogenesis of miRNAs is largely determined by the efficiency and fidelity of primary microRNA (pri-miRNA) processing by Microprocessor. Here, we investigated the roles of a secondary RNA element, an RNA bulge, in pri-miRNA processing. We discovered that the 3p-strand bulges in positions 7-9 from the Microprocessor cleavage sites (midB_7-9) contributes to determining the cleavage sites of Microprocessor, the 5p- and 3p-strand bugles in positions 10-12 (midB_10-12) blocked the unproductive cleavage, and the 3p-strand bulges in positions 6-7 (seedB) inhibited the productive cleavage of Microprocessor. more...

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL18573
15 Samples

Download data: FASTA, TXT

Series

Accession:: GSE158060

ID:: 200158060

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Select item 2001389507.

Single cut products of Microprocessor on pri-miRNAs

(Submitter supplied) The human Microprocessor complex cleaves primary microRNA (miRNA) transcripts (pri-miRNAs) to initiate miRNA synthesis. Microprocessor consists of DROSHA (an RNase III enzyme), and DGCR8. DROSHA has two conserved RNase III domains, which make double cuts on each of pri-miRNA strands. In this study, we show that Microprocessor has an unexpected single-cut activity, which creates a single cut on just one of the pri-miRNA strands using one of the two RNase III domains of DROSHA. more...

Organism:: synthetic construct

Type:: Other

Platforms:: GPL26697 GPL19424
2 Samples

Download data: TXT

Series

Accession:: GSE138950

ID:: 200138950

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Select item 2001114318.

Microprocessor depends on hemin to recognize the apical loop of primary microRNA

(Submitter supplied) Microprocessor, which consists of a ribonuclease III DROSHA and its cofactor DGCR8, initiates microRNA (miRNA) maturation by cleaving primary miRNA transcripts (pri-miRNAs). We recently demonstrated that the DGCR8 dimer recognizes the apical elements of pri-miRNAs, including the UGU motif, to accurately locate and orient Microprocessor on pri-miRNAs. However, the mechanism underlying the selective RNA binding remains unknown. more...

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL15520
17 Samples

Download data: CSV

Series

Accession:: GSE111431

ID:: 200111431

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Select item 2001421409.

High-throughput in vitro DROSHA processing on human pri-miRNA variants

(Submitter supplied) The in vitro high-throughput human pri-miRNA processing assays were conducted to check whether mismatches and wobble base pairs in the upper stem of pri-miRNAs affects the DROSHA cleavage.

Organism:: synthetic construct

Type:: Other

Platform:: GPL19424
6 Samples

Download data: TXT

Series

Accession:: GSE142140

ID:: 200142140

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20014213610.

The effect of mismatches and wobble base pairs in the upper stem of pri-miRNAs on DROSHA cleavage using small RNA sequencing

(Submitter supplied) The experiments were conducted to check whether mismatches and wobble base pairs in the upper stem of pri-miRNAs affects the DROSHA cleavage and thereby miRNA biogenesis.

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL16791
4 Samples

Download data: TXT

Series

Accession:: GSE142136

ID:: 200142136

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Select item 20013163611.

High-throughput in vitro DROSHA processing on artificial variants

(Submitter supplied) MicroRNAs (miRNAs) are small RNAs that regulate gene expression. miRNAs are produced from primary miRNAs (pri-miRNAs), the cleavage of which is catalyzed by the Microprocessor complex. Microprocessor therefore plays a key role in determining the efficiency and precision of miRNA production, and thus the function of the final miRNA product. In this study, we utilized high-throughput sequencing-integrated enzymology with purified Microprocessor proteins and randomized pri-miRNA sequences to investigate the catalytic mechanism of Microprocessor. more...

Organism:: synthetic construct

Type:: Other

Platform:: GPL26697
9 Samples

Download data: TXT

Series

Accession:: GSE131636

ID:: 200131636

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Select item 20006021312.

m6A-seq nuclear RNA

(Submitter supplied) To analyze the nuclear RNAs modified by the m6A mark

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
3 Samples

Download data: BED

Series

Accession:: GSE60213

ID:: 200060213

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Select item 20006021213.

METTL3 HITS-CLIP

(Submitter supplied) To identify METTL3 binding sites on endogenous nuclear RNAs, we performed HITS-CLIP endogenous METTL3

Organism:: Homo sapiens

Type:: Other

Platform:: GPL11154
3 Samples

Download data: BED

Series

Accession:: GSE60212

ID:: 200060212

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20021601214.

The high-throughput pri-miRNA cleavage assays on 262144 variants

(Submitter supplied) Microprocessor (MP), DROSHA-DGCR8, processes primary miRNA transcripts (pri-miRNAs) to initiate miRNA biogenesis. The canonical cleavage mechanism of MP has been extensively investigated and comprehensively validated for two decades. However, this canonical mechanism cannot account for the processing of certain pri-miRNAs in animals. In this study, by conducting high-throughput pri-miRNA cleavage assays for approximately 260,000 pri-miRNA sequences, we discovered and comprehensively characterized a noncanonical cleavage mechanism of MP. more...

Organism:: synthetic construct

Type:: Other

Platform:: GPL26526
8 Samples

Download data: TXT

Series

Accession:: GSE216012

ID:: 200216012

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Select item 20021579615.

Small RNA sequencing of DROSHA-KO HCT116 cells transfected with DROSHA wild type and mutants

(Submitter supplied) The experiments were conducted to examine the effects of DROSHA mutations on miRNA biogenesis

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL18573
12 Samples

Download data: TXT

Series

Accession:: GSE215796

ID:: 200215796

PubMed Similar studies

Select item 20021222916.

The high-throughput cleavage assays on 137 C.elegans pri-miRNAs

(Submitter supplied) Microprocessor (MP), cleaving primary microRNAs (pri-miRNAs) to initiate the biogenesis of thousands of miRNAs, was discovered in animals in 2004. Understanding the molecular mechanism of MP is critical for interpreting the gene-silencing roles of miRNAs in various cellular processes and human diseases. Though the molecular mechanism of human MP (hMP) has been comprehensively investigated for nearly two decades and is well understood, that of Caenorhabditis elegans MP (cMP, cDrosha-Pasha complex) is still unknown. more...

Organism:: Caenorhabditis elegans

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL26672
6 Samples

Download data: TXT

Series

Accession:: GSE212229

ID:: 200212229

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Select item 20007785617.

HP1BP3, a chromatin retention factor for co-transcriptional microRNA processing

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL11154
10 Samples

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Series

Accession:: GSE77856

ID:: 200077856

PubMed Full text in PMC Similar studies

Select item 20007785518.

HP1BP3, a chromatin retention factor for co-transcriptional microRNA processing (miRNA-Seq)

(Submitter supplied) Identification and characterization of HP1BP3 (a human histone H1 homologue) as a novel chromatin retention factor essential for the co-transcriptional processing of pri-miRNA.

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL11154
4 Samples

Download data: XLS

Series

Accession:: GSE77855

ID:: 200077855

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20007785419.

HP1BP3, a chromatin retention factor for co-transcriptional microRNA processing (ChIP-Seq)

(Submitter supplied) Identification and characterization of HP1BP3 (a human histone H1 homologue) as a novel chromatin retention factor essential for the co-transcriptional processing of pri-miRNA.