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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 13, 2018 |
| Title |
High-throughput in vitro DROSHA processing on 38,880 pri-miRNA variants |
| Organism |
synthetic construct |
| Experiment type |
Other
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| Summary |
To investigate the precise processing mechanism of pri-miRNA, we prepared 38,880 pri-miRNA variants which contain unique barcode sequences (Fang and Bartel, Mol Cell, 60: 131) and performed high-throughput processing experiments.
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| Overall design |
pri-miRNA pools which contain various sequence or structural elements were processed by recombinant DROSHA and DGCR8 proteins. Using the unique barcode information, cleavage sites per each pri-miRNA variant were identified and quantified.
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| Contributor(s) |
Kwon SC, Woo JS, Kim VN |
| Citation(s) |
30554947 |
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| Submission date |
Jul 24, 2018 |
| Last update date |
Mar 14, 2019 |
| Contact name |
S. Chul Kwon |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Hong Kong
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| Department |
School of Biomedical Sciences
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| Street address |
L1-05, Laboratory Block, 21 Sassoon Road, Pokfulam
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| City |
Hong Kong |
| State/province |
NA |
| ZIP/Postal code |
NA |
| Country |
Hong Kong |
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| Platforms (1) |
| GPL19604 |
Illumina HiSeq 2500 (synthetic construct) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA482630 |
| SRA |
SRP155016 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE117600_RAW.tar |
688.1 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE117600_designed_variants.txt.gz |
105.1 Kb |
(ftp)(http) |
TXT |
| GSE117600_variant_counts.txt.gz |
924.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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