GEO DataSets

(Submitter supplied) The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. To find leads for maturation markers we performed a pilot deep-sequencing transcriptome analysis of ovarian tissue derived from a yellow eel, a prepubertal silver eel and a post-spawning matured eel. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20639

3 Samples

Download data: XLSX

(Submitter supplied) The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. Three-year-old farmed silver female European eels were injected with 20 mg salmon pituitary extract (SPE) once per week and sampled after 4 weekly hormone injections. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20640

6 Samples

Download data: XLSX

(Submitter supplied) Gene expression analyses have been performed on liver tissue of sexually mature and immature males using microarrays. 60 eels were transferred to two independent temperature controlled recirculation water units connected to two 500 L cylindro-conical tanks (30 fish per tank) where the fish were acclimated to seawater (35 PSU salinity) over a 2 week period.Eel males in one of the seawater units were injected intramuscularly every week over a 140 day period with 2000 IU hCG/kg (human chorionic gonadotropin, Sigma–Aldrich Chemical) dissolved in 0.9% saline to induce sexual maturation. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

8 Samples

Download data: TXT

(Submitter supplied) Gene expression analyses have been performed on brain tissue of sexually mature and immature males using microarrays. 60 eels were transferred to two independent temperature controlled recirculation water units connected to two 500 L cylindro-conical tanks (30 fish per tank) where the fish were acclimated to seawater (35 PSU salinity) over a 2 week period.Eel males in one of the seawater units were injected intramuscularly every week over a 140 day period with 2000 IU hCG/kg (human chorionic gonadotropin, Sigma–Aldrich Chemical) dissolved in 0.9% saline to induce sexual maturation. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

8 Samples

Download data: TXT

(Submitter supplied) An European eel-specific microarray platform was developed to identify genes involved in response to pollutants. A comparative analysis of gene expression was conducted between European eel Anguilla anguilla individuals from lowly-polluted Wijmeers pond at Uitbergen (Belgium), highly-polluted Hazewinkel pond at Willebroek (Belgium), extremely-polluted Dessel-Schotel canal at the locations of Schotel (Belgium) and low polluted Bolsena lake (Italy) environments.

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

38 Samples

Download data: TXT

(Submitter supplied) Nanometric revolution is underway, promising technical innovations in a wide range of applications, leading to a potential boost in environmental discharges. Nanoparticle propensity to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary consumers while a lack of knowledge for higher trophic levels persists. This study focused on a predatory fish, the European eel Anguilla anguilla exposed to gold nanoparticles (AuNP, 10 nm, PEG-coated) for 21 days at three concentration levels in food: 0 (NP0), 1 (NP1) and 10 (NP10) mg Au.kg-1 . more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24571

24 Samples

Download data: CSV, FASTA

(Submitter supplied) This study aimed at providing insights into the hypothesized functional link between olfactory sensing of the spawning ground and final sexual maturation. We have therefore assessed the presence and expression levels of olfactory genes by RNA sequencing (RNAseq) of the olfactory rosettes in homing chum salmon Oncorhynchus keta Walbaum from the coastal sea to 75 km upstream the rivers at the pre-spawning ground. more...

Organism:

Oncorhynchus keta

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20852

12 Samples

Download data: FA, XLSX

(Submitter supplied) Since the identification of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the main maturation-inducing hormone (MIH) in Japanese eel A. japonica, female European eels have been injected with this steroid to induce oocyte maturation and ovulation. However, the females injected with DHP need to ovulate rapidly following the DHP injection to release good quality eggs. In this study, we compared treatment of progesterone (P) with DHP treatment as control, in vitro, to determine dose-response effects, and in vivo, at a single dose. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28518

12 Samples

Download data: XLSX

(Submitter supplied) This project aims to study the role played by small non-coding RNAs in the response of aquatic organisms to the presence of micropollutants in the environment. MiRNA were purified from Eels (Anguilla anguilla) sampled from two sites along the Gironde aquatic system with contrasted pollution profiles.

Organism:

Anguilla anguilla

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24544

12 Samples

Download data: CSV

(Submitter supplied) An European eel-specific microarray platform was developed to identify genes involved in response to pollutants

Organism:

Anguilla anguilla

Type:

Expression profiling by array

Platform:

GPL15124

23 Samples

Download data: TXT

(Submitter supplied) We investigated transcriptional and translational changes in rete mirabile tissue of European yellow and silver eels. We therefore analyzed the transcriptome as well as the proteome of rete capillaries of specimens of both developmental stages. The results clearly show the presence of a large number of membrane transport proteins in the transcriptome as well as in the proteome. ATP required to energize ion and metabolite transport can be generated in the aerobic metabolism. more...

Organism:

Anguilla anguilla

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20640 GPL28518

11 Samples

Download data: FA, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.This contains multi-omics datasets transcriptomic (RNA-Seq), methylomic (WGBS), and epigenomics (ATAC-seq) obtained during onset of sexual maturation in Atlantic salmon. We used gene regulatory networks (GRNs) to integrate results from these multi-omic analyses to identify key regulators of maturation.

Organism:

Salmo salar

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:

GPL23792 GPL24981 GPL28848

88 Samples

Download data: TSV

(Submitter supplied) We sequenced mRNA from four biological replicates of each tissue before (T1) and after the onset of maturation (T2, T3, T4). A total of 3.2 billion paired-end reads were mapped against the Atlantic salmon reference genome with 72% mapping efficiency to create an average depth of 50 million reads per library. The number of differentially expressed genes (DEGs) increased with elapsed time following the onset of the long light photoperiod for the two BPG axis tissues (pituitary and ovary). more...

Organism:

Salmo salar

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24981

64 Samples

Download data: TXT

(Submitter supplied) We sequenced 12 ATAC-Seq lbraries from four biological replicates before (T1) and after the onset of maturation (T2, T3, T4) from liver. To characterise changes in chromatin state following long light initiation, we defined differentially accessible regions (DARs) where mapping counts differed significantly between T1 and other time points. This revealed a strong early remodelling in the chromatin state landscape, as most DARs were observed at T2 (n=1501) before decreasing in stepwise fashion at T3 (n=477) and T4. more...

Organism:

Salmo salar

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL28848

12 Samples

Download data: TXT

(Submitter supplied) We performed a time course experiment, whereby animals were manipulated with photoperiod before tissues were collected across the time window when animals commence sexual development. We performed whole genome bisulfite sequencing of three salmon tissues (pituitary, ovary and liver) at both the beginning and end of the experiment, to take a first look at the patterns of DNA methylation and examine how they change in response to the onset of an important life history trait. more...

Organism:

Salmo salar

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL23792

12 Samples

Download data: TSV