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| Status |
Public on Aug 27, 2015 |
| Title |
The olfactory transcriptome and progression of sexual maturation in homing chum salmon Oncorhynchus keta |
| Organism |
Oncorhynchus keta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study aimed at providing insights into the hypothesized functional link between olfactory sensing of the spawning ground and final sexual maturation. We have therefore assessed the presence and expression levels of olfactory genes by RNA sequencing (RNAseq) of the olfactory rosettes in homing chum salmon Oncorhynchus keta Walbaum from the coastal sea to 75 km upstream the rivers at the pre-spawning ground. RNAseq revealed the expression of 75 known and 27 unknown salmonid olfactory genes of which 13 genes were differentially expressed between fish from the pre-spawning area and from the coastal area, suggesting an important role of these genes in homing. Olfactomedins and ependymin are candidates among the differentially expressed genes that may connect olfactory reception to the expression of sgnrh to regulate final maturation.
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| Overall design |
Deep-sequencing transcriptome analysis of twelve chum salmon olfactory rosette RNA samples: three females and three males from the pre-spawning area and three females and three males from the coastal area.
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| Contributor(s) |
Palstra AP, Dirks RP, Planas JV, Ueda H |
| Citation(s) |
26397372 |
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| Submission date |
Aug 26, 2015 |
| Last update date |
Apr 01, 2020 |
| Contact name |
Ron P Dirks |
| E-mail(s) |
[email protected]
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| Phone |
+31-71-2074610
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| Organization name |
Future Genomics Technologies BV
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| Street address |
Sylviusweg 74
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| City |
Leiden |
| ZIP/Postal code |
2333 BE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL20852 |
Illumina HiSeq 2500 (Oncorhynchus keta) |
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| Samples (12)
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| GSM1861765 |
Ishikari Bay, male 1, OE I2 |
| GSM1861766 |
Ishikari Bay, male 2, OE I5 |
| GSM1861767 |
Ishikari Bay, male 3, OE I7 |
| GSM1861768 |
Prespawning ground, female 1, OE P2 |
| GSM1861769 |
Prespawning ground, female 2, OE P3 |
| GSM1861770 |
Prespawning ground, female 3, OE P4 |
| GSM1861771 |
Prespawning ground, male 1, OE P7 |
| GSM1861772 |
Prespawning ground, male 2, OE P9 |
| GSM1861773 |
Prespawning ground, male 3, OE P10 |
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| Relations |
| BioProject |
PRJNA293937 |
| SRA |
SRP062862 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72390_DiffExpr_Location_7-12_vs_1-6_Oncorhynchus_keta_de_novo.xlsx |
17.7 Mb |
(ftp)(http) |
XLSX |
| GSE72390_DiffExpr_Location_7-12_vs_1-6_Salmo_salar_3_mismatches.xlsx |
4.9 Mb |
(ftp)(http) |
XLSX |
| GSE72390_Oncorhynchus_keta_cDNA.fa.gz |
22.6 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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