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| Status |
Public on Aug 28, 2020 |
| Title |
Multitissue DNA methylome profiling during onset of salmon maturation |
| Organism |
Salmo salar |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
We performed a time course experiment, whereby animals were manipulated with photoperiod before tissues were collected across the time window when animals commence sexual development. We performed whole genome bisulfite sequencing of three salmon tissues (pituitary, ovary and liver) at both the beginning and end of the experiment, to take a first look at the patterns of DNA methylation and examine how they change in response to the onset of an important life history trait. Comparison across timepoints revealed 6,373 differentially methylated regions (DMRs), of which approximately 50% were located within genes (DMGs). The ovary underwent the most profound remodelling, with a strong bias towards increased methylation levels (hyper-methylation). Weak correlation was observed considering all available genes, suggesting methylation may not be the key epigenomic regulator of global expression in the context of our experiment. However, we found a significant overlap between DMGs and differentially expressed genes in the ovary. Taken together, our results suggest chromatin remodelling genes play a role in the commitment of animals to the sexual maturation pathway.
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| Overall design |
We sequenced 12 WGBS libraries of three tissues (pituitary gland, ovary and liver) at two timepoints before (T1) and after the onset of maturation (T4).
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| Contributor(s) |
Mohamed A, Naval-Sanchez M, Menzies M, Evans B, Reverter A, Kijas J |
| Citation(s) |
35650521 |
| |
| Submission date |
Aug 27, 2020 |
| Last update date |
Jun 21, 2022 |
| Contact name |
Amin Roushdy Mohamed |
| E-mail(s) |
[email protected]
|
| Organization name |
CSIRO
|
| Department |
Agriculture and Food
|
| Lab |
Aquaculture Genomics
|
| Street address |
306 Carmody Rd, University of Queensland
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
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| Platforms (1) |
| GPL23792 |
Illumina HiSeq 2500 (Salmo salar) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE157003 |
Multiomics profiling during onset of Salmon maturation |
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| Relations |
| BioProject |
PRJNA659747 |
| SRA |
SRP279074 |
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