GEO DataSets

(Submitter supplied) In this study, we employed a combination of RIP-seq and short- and long-wave iCLIP technologies to identify transcripts associated with cytoplasmic RNPs containing the RNA-binding protein Drosophila Imp. We also made a Imp knockdown vs luciferase control experiment.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

16 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL17639 GPL13222

45 Samples

Download data: BED

(Submitter supplied) A key function for RNA-binding proteins in orchestrating plant development and environmental responses is well established. However, the lack of a genome-wide view on their in vivo regulatory landscapes represents a gap in understanding the mode of action of plant RNA-binding proteins. Here, we conducted RNAseq to determine the genome-wide regulation repertoire of the circadian clock-regulated Arabidopsis thaliana glycine-rich RNA-binding protein AtGRP7.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13222

18 Samples

Download data: TSV

(Submitter supplied) A key function for RNA-binding proteins in orchestrating plant development and environmental responses is well established. However, the lack of a genome-wide view on their in vivo binding targets and binding landscapes represents a gap in understanding the mode of action of plant RNA-binding proteins. Here, we conducted RNA Immunoprecipitation (RIP-seq) for genome-wide determining the binding repertoire of the circadian clock-regulated Arabidopsis thaliana glycine-rich RNA-binding protein AtGRP7.

Organism:

Arabidopsis thaliana

Type:

Other

Platform:

GPL13222

6 Samples

Download data: TSV

(Submitter supplied) A key function for RNA-binding proteins in orchestrating plant development and environmental responses is well established. However, the lack of a genome-wide view on their in vivo binding targets and binding landscapes represents a gap in understanding the mode of action of plant RNA-binding proteins. Here, we adapt individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) for genome-wide determining the binding repertoire of the circadian clock-regulated Arabidopsis thaliana glycine-rich RNA-binding protein AtGRP7. more...

Organism:

Arabidopsis thaliana

Type:

Other

Platform:

GPL17639

21 Samples

Download data: BED

(Submitter supplied) Mutant embryos lacking maternal and zygotic HOW exhibit defects in mesoderm development. How is an RNA binding protein that regulates the levels of mRNAs by controling RNA metabolism. To gain an insight as to the profile of mRNAs regulated by HOW during early mesoderm development we analyzed the array of mRNAs whose levels are altered in the how mutant embryos. Keywords: Genetic modification

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS3255

Platform:

GPL72

4 Samples

Download data: CEL, CHP

Analysis of mutant embryos lacking both maternal and zygotic Held out wing (HOW), an RNA-binding protein (RBP) highly expressed in the mesoderm during early embryogenesis. how mutant embryos exhibit aberrant mesoderm spreading. Results provide insight into the role of HOW in mesoderm development.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL72

Series:

GSE7772

4 Samples

Download data: CEL, CHP

(Submitter supplied) Staufen-associated mRNAs were identified in early Drosophila embryos by RNA-co-immunoprecipitation followed by microarray analysis (RIP-Chip), using two complementary approaches: RIP-Chip from wild-type embryos using a synthetic anti-Staufen antibody, and RIP-Chip from transgenic GFP-Staufen-expressing embryos using an anti-GFP antibody. Genome-wide transcript expression in whole embryos was also assessed for the wild-type and GFP-Staufen lines.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL10539

18 Samples

Download data: PAIR

(Submitter supplied) Purpose: Communication between growth cones and their environment plays a central role in assembling neural circuits. We use Tandemly-Tagged Ribosome Affinity Purification (T-TRAP) of mRNA from R cells followed by RNA-seq for multiple time points during development to follow gene expression during target selection and synapse formation. Methods: We chose a ribosome trap method by modifying the N-terminus of the Drosophila ribosomal protein RpL10 with two tandemly arranged epitopes, 3X FLAG and GFP, separated by the Tobacco Etch Virus (TEV) protease site and expressed this in specific cell types using the GAL4/UAS system. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

14 Samples

Download data: TXT

(Submitter supplied) RNA were extracted from 5 adult male and 5 adult female dU2AF50 wt and mutant flies grown at either the permisive (25C) or restrictive (30C) temperature for 4 days. Keywords: other

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS664

Platform:

GPL72

12 Samples

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(Submitter supplied) Nuclear and cytoplasmic RNA were extracted by hypotonic cell lysis from D.-mel2 cell knock down by RNAi against dU2AF50 or with non-specific dsRNA. Keywords: other

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS667

Platform:

GPL72

12 Samples

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(Submitter supplied) Genome wide identification of the RNAs associated with the Drosophila large U2AF subunit Keywords: repeat sample

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1203

5 Samples

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Examination of cytoplasmic and nuclear mRNA distribution in D.mel2 cell line after RNAi knock-down of dU2AF50 (large subunit of pre-mRNA splicing factor U2AF). dU2AF50 knock-down analyzed in reference to non-specific RNAi knock-down.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 other, 2 protocol sets

Platform:

GPL72

Series:

GSE1344

12 Samples

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Analysis of mutant defective in dU2AF50 (large subunit of pre-mRNA splicing factor U2AF). Mutant examined after growth at permissive (25°C) and restrictive (30°C) temperatures in reference to wild type.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 genotype/variation, 2 temperature sets

Platform:

GPL72

Series:

GSE1345

12 Samples

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(Submitter supplied) The accompanying dataset is the result of a systematic study to identify the RNA cargoes associated with the cytoskeletal motor proteins of Saccharomyces cerevisiae. We immunopurified, via the use of integrated, C-terminal GFP and 9Myc tags, the five actomyosin motors, Myo1, Myo2, Myo3, Myo4, and Myo5; the kinesin-like proteins Kar3, Kip1, Kip2, Kip3, and Smy1; and the dynein, Dyn1, from S. cerevisiae. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

4 related Platforms

118 Samples

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