|
| Status |
Public on Sep 28, 2017 |
| Title |
Identification of AtGRP7 targets by iCLIP |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
|
| Summary |
A key function for RNA-binding proteins in orchestrating plant development and environmental responses is well established. However, the lack of a genome-wide view on their in vivo binding targets and binding landscapes represents a gap in understanding the mode of action of plant RNA-binding proteins. Here, we adapt individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) for genome-wide determining the binding repertoire of the circadian clock-regulated Arabidopsis thaliana glycine-rich RNA-binding protein AtGRP7. We have established iCLIP for plants to identify target transcripts of the RNA-binding protein AtGRP7. This paves the way to investigate the dynamics of posttranscriptional networks in response to exogenous and endogenous cues.
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| |
|
| Overall design |
AtGRP7 iCLIP
|
| |
|
| Contributor(s) |
Meyer K, Köster T, Nolte C, Weinholdt C, Lewinski M, Grosse I, Staiger D |
| Citation(s) |
29084609 |
| |
| Submission date |
May 30, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Martin Lewinski |
| Organization name |
Bielefeld University
|
| Department |
RNA Biology and Molecular Physiology
|
| Street address |
Universitätsstraße 25
|
| City |
Bielefeld |
| ZIP/Postal code |
33615 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
|
| Samples (21)
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| This SubSeries is part of SuperSeries: |
| GSE99616 |
Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7 |
|
| Relations |
| BioProject |
PRJNA388445 |
| SRA |
SRP108277 |
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