GEO DataSets

(Submitter supplied) Brown adipose tissue is specialized to burn lipids for heat generation as a natural defense against cold and obesity. Previous studies established microRNAs as essential regulators of brown adipocyte differentiation, but it remains unknown whether microRNAs are required for the feature maintenance of mature brown adipocytes. To address this question, we ablated Dgcr8, a key regulator of the microRNA biogenesis pathway, in mature brown as well as white adipocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: XLS

(Submitter supplied) Brown adipocytes are specialized for heat generation and energy expenditure as a defense against cold and obesity. Recent studies demonstrate that brown adipocytes arise in vivo from a Myf5-positive, myoblastic progenitor by the action of PRDM16. Here, we identified a brown fat-enriched miRNA cluster mir-193b-365 as a key regulator of brown fat development. Blocking miR-193b and/or miR-365 in primary brown preadipocytes dramatically impaired brown adipocyte adipogenesis whereas myogenic markers were significantly induced. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8492

4 Samples

Download data: CEL

(Submitter supplied) We performed a genome-wide deep sequencing analysis of the microRNAs abundant in mesenchymal stem cells (MSCs) derived from murine brown adipose tissue and in in vitro differentiated mature brown adipocytes. Several microRNAs were identified as differentially regulated when comparing datasets from MSCs vs. mature fat cells. These microRNAs may have an implication in the regulation of adipogenesis as well as thermogenesis in brown adipose tissue (BAT).

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9273

2 Samples

Download data: TXT

(Submitter supplied) microRNAs levels were measured in brown adipose tissue of male C57Bl6N mice that were kept at RT or during cold exposure (8°C) for 24 hrs. Several miRNAs including myomirs were identified to be regulated in response to cold.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL7732

8 Samples

Download data: TXT

(Submitter supplied) To systemically determine the translational control of gene expression in adipose, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

24 Samples

Download data: XLSX

(Submitter supplied) Multipotent C3H10T1/2 cells can be induced to differentiate into mature brown adipocytes by 3-days BMP7 pretreatment followed by standard adipogenic induction.

Organism:

Homo sapiens; Mus musculus; Rattus norvegicus

Type:

Non-coding RNA profiling by array

Platform:

GPL20722

2 Samples

Download data: TXT

(Submitter supplied) Multipotent C3H10T1/2 cells can be induced to differentiate into mature brown adipocytes by 3-days BMP7 pretreatment followed by standard adipogenic induction. We used microarrays to detail the global programme of gene expression in C3H10T1/2 cells after 3-day BMP7 (3.3 nM) treatment.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

8 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6244 GPL570 GPL13607

58 Samples

Download data: CEL, TXT

(Submitter supplied) The utility of human pluripotent stem cells as a tool for understanding disease and as a renewable source of cells for transplantation therapies is dependent on efficient differentiation protocols that convert these cells into relevant adult cell types. Here we report the robust and efficient differentiation of human pluripotent stem cells into adipocytes. We found that inducible expression of PPARG2 in pluripotent stem cell-derived mesenchymal progenitor cells programmed their development towards an adipocyte cell fate. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13607

24 Samples

Download data: TXT

(Submitter supplied) The utility of human pluripotent stem cells as a tool for understanding disease and as a renewable source of cells for transplantation therapies is dependent on efficient differentiation protocols that convert these cells into relevant adult cell types. Here we report the robust and efficient differentiation of human pluripotent stem cells into adipocytes. We found that inducible expression of PPARG2 in pluripotent stem cell-derived mesenchymal progenitor cells programmed their development towards an adipocyte cell fate. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL6244

34 Samples

Download data: CEL, TXT

(Submitter supplied) Cre recombinase activity was induced in differentiating brown adipocytes from CreERT2 Sykflox/flox mice in vitro, which resulted in a partial loss of Syk protein in mature brown adipocytes. Such cells were viable, morphologically normal and displayed largely normal gene expression as indicated by mRNA sequencing and qPCR analysis, suggesting that Syk is not required for survival and gene expression of terminally differentiated brown adipocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: TXT

(Submitter supplied) Effects of YBX1 activation in PPARγ-indcuded C3H/10T1/2-SAM pre-adipocytes on the transcriptome of cells during early differentation stages

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

51 Samples

Download data: TSV

(Submitter supplied) Here we provide scRNAseq data from the stromal vascular fraction of adult (8 weeks) murine brown adipose tissue. This allows studying cellular composition and cellular heterogeneity within brown fat.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

1 Sample

Download data: MTX, TSV

(Submitter supplied) BAT obtained from embryos at E14.5, E15.5 or E16.5 of C57Bl6J mice used to prepare RNA which was then processed for analysis using MoGene-2_1-st Affymetrix microarrays according to standard procedures.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL25793

9 Samples

Download data: CEL

(Submitter supplied) The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Keywords: tissue comparison from mice

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2813

Platform:

GPL1261

6 Samples

Download data: CEL, CHP, DCP, TXT

Comparison of brown and white adipose tissues. Brown fat cells are specialized to dissipate energy and can counteract obesity. Results provide insight into the molecular mechanisms controlling brown fat cell determination.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL1261

Series:

GSE8044

6 Samples

Download data: CEL, CHP, DCP, TXT

(Submitter supplied) We report molecular characterization of human brown and white adipocytes. We showed that PAZ6 and SW872 cells exhibit classical molecular and phenotypic markers of brown and white adipocytes, respectively. However, SGBS cells presented a versatile phenotype of adipocyte

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

7 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Other

Platforms:

GPL17021 GPL21103 GPL19057

20 Samples

Download data

(Submitter supplied) The absence of thousands of recently annotated small open reading frame (smORF)-encoded peptides and small proteins (microproteins) from databases has precluded their analysis in metabolism and metabolic disease. Given the outsized importance of small proteins and peptides in metabolism—insulin, leptin, amylin, glucagon, and glucagon-like peptide-1 (GLP-1)—microproteins are a potentially rich source of uncharacterized metabolic regulators. more...

Organism:

Mus musculus

Type:

Other

Platform:

GPL19057

14 Samples

Download data: GTF

(Submitter supplied) The absence of thousands of recently annotated small open reading frame (smORF)-encoded peptides and small proteins (microproteins) from databases has precluded their analysis in metabolism and metabolic disease. Given the outsized importance of small proteins and peptides in metabolism—insulin, leptin, amylin, glucagon, and glucagon-like peptide-1 (GLP-1)—microproteins are a potentially rich source of uncharacterized metabolic regulators. more...

Organism:

Mus musculus

Type:

Other

Platforms:

GPL17021 GPL21103

6 Samples

Download data: XLSX