GEO DataSets

(Submitter supplied) We report the genome wide DNA binding patterns of wild type FOXK1 in asynchronous HeLa cells using chromatin precipitation followed by high-throughput sequencing (ChIP-seq). We find that FOXK1 is bound to the promoter of a number of cell cycle genes including the E2F binding partner TFDP1. This study is the first reported study of FOXK1 binding on a genome-wide scale in HeLa cells.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

2 Samples

Download data: BED

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL4133 GPL9115

93 Samples

Download data: BED

(Submitter supplied) We report the genome wide DNA binding patterns of wild type FOXM1 in asynchronous HeLa cells using chromatin precipitation followed by high-throughput sequencing (ChIP-seq). We find that FOXM1 is bound to the promoter of a number of cell cycle genes including PLK, AURKB, and CCNB1.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

2 Samples

Download data: BED

(Submitter supplied) We identified the cell cycle-regulated mRNA transcripts genome-wide in the osteosarcoma derived U2OS cell line. This resulted in 2,140 transcripts mapping to 1,871 unique cell cycle-regulated genes that show periodic oscillations across multiple synchronous cell cycles. We identified genomic loci bound by the G2/M transcription factor FOXM1 by Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) and associated these with cell cycle-regulated genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

91 Samples

Download data

(Submitter supplied) There are nearly fifty forkhead (FOX) transcription factors encoded in the human genome and due to sharing a common DNA binding domain, they are all thought to bind to similar DNA sequences. It is therefore unclear how these transcription factors are targeted to specific chromatin regions to elicit specific biological effects. Here we used ChIP-seq to investigate the genome-wide chromatin binding mechanisms used by the forkhead transcription factor FOXM1. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

3 Samples

Download data: BED

(Submitter supplied) COUP-TFII plays a critical role in angiogenesis during development. It has also been shown to suppress Notch signaling pathway to confer vein identity. However, the downstream targets and the mechanism mediate COUP-TFII function to regulate these processes remain elusive. To identify the downstream targets and the mechanism by which COUP-TFII regulates agiogenesis and vein specification, we knocked down COUP-TFII in HUVEC cells using COUP-TFII specific siRNA and used microarray analysis to identify downstream targets. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, TXT

(Submitter supplied) The Forkhead transcription factor, FOXM1, is a key regulator of the cell cycle and is over-expressed in most types of cancer. FOXM1, similar to other Forkhead (FKH) factors binds to a canonical FKH motif in vitro. However, genome-wide mapping studies in different cell lines have shown a lack of enrichment of the FKH motif at FOXM1 binding sites suggesting an alternative mode of chromatin recruitment. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11154

15 Samples

Download data: BED

(Submitter supplied) ChIP-seq analysis demonstrates FoxK1 binding to proximal promoters and enhancers, especially to genes containing a TGTTTAC motif, which is similar to the FoxA/FoxO motifs.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

12 Samples

Download data: BIGWIG, NARROWPEAK

(Submitter supplied) NO effects on gene expression, independent of cGMP, were examined globally. Differentiated human U937 cells that lack soluble guanylate cyclase were exposed to Snitrosoglutathione, a NO donor, or glutathione without or with dibutyryl-cAMP (Bt2cAMP). NO regulated 110 transcripts that annotated disproportionately to the cell cycle and cell proliferation (47/110, 43%) and more frequently than expected contained AU-rich, post-transcriptional regulatory elements (ARE). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1645

Platform:

GPL80

42 Samples

Download data

Analysis of soluble guanylate cyclase-deficient U937 cells exposed to the nitric oxide (NO) donor S-nitrosoglutathione. Cells treated in the presence of dibutyryl-cAMP (Bt2cAMP) to block signaling due to NO-induced decreases in cAMP. Results provide insight into the cGMP-independent effects of NO.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 agent, 2 development stage sets

Platform:

GPL80

Series:

GSE2649

42 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL11532 GPL16570 GPL17586

87 Samples

Download data: CEL

(Submitter supplied) Transcriptional programmes involved in the eukaryotic cell cycle are activated sequentially throughout the process. In particular, the set of genes required for S and G2-M phases are highly conserved and induced one after the other. We used gene expression microarrays to analyse the global programme of gene expression of human primary fibroblasts from a quiescence state (G0) to a synchronised cell proliferation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

23 Samples

Download data: CEL, TXT

(Submitter supplied) Transcriptional programmes involved in the eukaryotic cell cycle are activated sequentially throughout the process. In particular, the set of genes required for S and G2-M phases are highly conserved and induced one after the other. We used gene expression microarrays to analyse the global programme of gene expression of human primary fibroblasts from a quiescence state (G0) to a synchronised cell proliferation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11532

16 Samples

Download data: CEL, TXT

(Submitter supplied) Transcriptional programmes involved in the eukaryotic cell cycle are activated sequentially throughout the process. In particular, the set of genes required for S and G2-M phases are highly conserved and induced one after the other. We used gene expression microarrays to analyse the global programme of gene expression of human primary fibroblasts from a quiescence state (G0) to a synchronised cell proliferation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11532

24 Samples

Download data: CEL, TXT

(Submitter supplied) Transcriptional programmes involved in the eukaryotic cell cycle are activated sequentially throughout the process. In particular, the set of genes required for S and G2-M phases are highly conserved and induced one after the other. We used gene expression microarrays to analyse the global programme of gene expression of mouse embryonic fibroblast from a quiescence state (G0) to a synchronised cell proliferation

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

24 Samples

Download data: CEL, TXT