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| Status |
Public on Oct 01, 2018 |
| Title |
Expression data of synchronised primary human fibroblasts (NHDF, Data Set 1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptional programmes involved in the eukaryotic cell cycle are activated sequentially throughout the process. In particular, the set of genes required for S and G2-M phases are highly conserved and induced one after the other.
We used gene expression microarrays to analyse the global programme of gene expression of human primary fibroblasts from a quiescence state (G0) to a synchronised cell proliferation
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| Overall design |
Primary human fibroblasts (NHDF) were firstly synchronised by limiting nutrients (from 10% to 0.1% of faetal calf serum) in the medium for 48 h. Samples were collected at t= 0 (after 48 h starvation but before full medium replacement), t = 6 h, t = 12 h, t = 18 h, t = 24 h, t = 30 h, t = 36 h, t = 42 h, t = 48 h. Three techincal replica were collected for time points 0 h, 24 h, 30 h, and controls (unsynchronised).
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| Contributor(s) |
Giotti B, Barnett MW, Chen D, Freeman TC |
| Citation(s) |
30452682 |
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| Submission date |
Oct 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Tom C Freeman |
| E-mail(s) |
[email protected]
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| Phone |
0131 6519203
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| Organization name |
Roslin Institute
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| Department |
Genetics and Genomics
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| Lab |
Freeman
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| Street address |
Easter Bush Campus, Midlothian
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| City |
Edinburgh |
| ZIP/Postal code |
EH25 9RG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM2804150 |
NHDF, 0 h_Rep1 (set1) |
| GSM2804151 |
NHDF, 0 h_Rep2 (set1) |
| GSM2804152 |
NHDF, 0 h_Rep3 (set1) |
| GSM2804153 |
NHDF, 6 h_Rep1 (set1) |
| GSM2804154 |
NHDF, 6 h_Rep2 (set1) |
| GSM2804155 |
NHDF, 12 h_Rep1 (set1) |
| GSM2804156 |
NHDF, 12 h_Rep2 (set1) |
| GSM2804157 |
NHDF, 18 h_Rep1 (set1) |
| GSM2804158 |
NHDF, 18 h_Rep2 (set1) |
| GSM2804159 |
NHDF, 24 h_Rep1 (set1) |
| GSM2804160 |
NHDF, 24 h_Rep2 (set1) |
| GSM2804161 |
NHDF, 24 h_Rep3 (set1) |
| GSM2804162 |
NHDF, 30 h_Rep1 (set1) |
| GSM2804163 |
NHDF, 30 h_Rep2 (set1) |
| GSM2804164 |
NHDF, 30 h_Rep3 (set1) |
| GSM2804165 |
NHDF, 36 h_Rep1 (set1) |
| GSM2804166 |
NHDF, 36 h_Rep2 (set1) |
| GSM2804167 |
NHDF, 42 h_Rep1 (set1) |
| GSM2804168 |
NHDF, 42 h_Rep2 (set1) |
| GSM2804169 |
NHDF, 48 h_Rep1 (set1) |
| GSM2804170 |
NHDF, 48 h_Rep2 (set1) |
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| This SubSeries is part of SuperSeries: |
| GSE104619 |
Expression data of synchronised mouse embryonic fibroblasts (MEF) and synchronised primary human fibroblasts (NHDF) |
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| Relations |
| BioProject |
PRJNA413367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104616_NHDF_1_matrix_data.txt.gz |
3.0 Mb |
(ftp)(http) |
TXT |
| GSE104616_RAW.tar |
94.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data are available on Series record |
| Processed data included within Sample table |
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