GEO DataSets

(Submitter supplied) The RNA-binding protein Hfq is a global regulator, which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) For transcript analysis of responses in Medicago truncatula to its symbiont Sinorhizobium meliloti wild type or the succinoglycan-deficient exoY mutant we compared transcripts from line A17 roots inoculated with 25 mL OD = 0.05 S. meliloti wild type or exoY mutant. Keywords: 1 line; 2 S. meliloti samples

Organism:

Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4799

6 Samples

Download data

(Submitter supplied) We characterized the transcriptome of a Sinorhizobium meliloti emmA insertion mutant strain.

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

6 Samples

Download data: CEL

(Submitter supplied) Publication title: Pseudonodule formation by wild type and symbiotic mutant Medicago truncatula in response to auxin transport inhibitors This SuperSeries is composed of the SubSeries listed below.

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platforms:

GPL9757 GPL4652

23 Samples

Download data: CEL

(Submitter supplied) We used an Affymetrix oligonucleotide microarray consisting of 9,935 tentative consensus (TC)sequences, which are based on cDNA libraries (Mitra et al., 2004 [PMID:15220482]). We examined gene expression of wild-type M. truncatula plants after inoculation with wild-type S. meliloti at 1 d, 4 d, 7d, 14 d, and 21 d. We chose these time points because they span the range of development of the Rhizobium-legume symbiosis, from initiation of the interaction through nitrogen fixation. more...

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

10 Samples

Download data: CEL, TXT

(Submitter supplied) Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. more...

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

4 Samples

Download data: CEL, TXT

(Submitter supplied) Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. more...

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

4 Samples

Download data: CEL, TXT

(Submitter supplied) Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. more...

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

6 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

65 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes for strains overexpressing each of the Sinorhizobium meliloti ECF sigma factors the via a plasmid-borne, melibiose-inducible promoter plasmid (PmelA; pCAP11: Pinedo et al. 2008 J Bacteriol 190:2947-2956) compared to control strains carrying the empty vector.

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL9757

45 Samples

Download data: CEL

(Submitter supplied) We characterized transcriptomes of Sinorhizobium meliloti root nodule bacteria with mutations in sigma factor genes.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

20 Samples

Download data: CEL

(Submitter supplied) The involvement of ROS in the legume – Rhizobium symbiotic interaction has been highlighted (Santos et al., 2001; Rubio et al., 2004). This interaction is characterized by the formation of a new organ on the root, the nodule and by the penetration, in parallel, of the bacteria into the root tissue via an infection thread (IT) (Parniske and Downie, 2003; Gage, 2004). H2O2 production has been shown in ITs during the Medicago – Sinorhizobium meliloti interaction (Santos et al., 2001). more...

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Genome variation profiling by SNP array

Platform:

GPL4652

8 Samples

Download data: CEL

(Submitter supplied) Within this work we identified and characterized SMc03169 (hhrA) as a new Sinorhizobium meliloti gene product with relevance to biological nitrogen fixation symbiosis with leguminous plants. HhrA belongs to the TetR-family of repressors and its deletion from S. meliloti genome affected considerably gene expression as well as several phenotypic traits.

Organism:

Medicago sativa; Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL4652

6 Samples

Download data: CEL

(Submitter supplied) Investigation of whole genome gene expression level changes in a Sinorhizobium meliloti 1021 rpoH1 rpoH2 double mutant, compared to the wild-type strain. The mutations engineered into this strain render it deficient in symbiotic nitrogen fixation. The mutants analyzed in this study are further described in Mitsui, H, T. Sato, Y. Sato, and K. Minamisawa. 2004. Sinorhizobium meliloti RpoH1 is required for effective nitrogen-fixing symbiosis with alfalfa. more...

Organism:

Sinorhizobium meliloti 1021

Type:

Expression profiling by array

Platform:

GPL19375

20 Samples

Download data: CALLS, PAIR

(Submitter supplied) The dual genome Affymetrix SymbiosisChip contains probe sets for S. meliloti ORFs annotated in 2001, intergenic regions over 150 bp, and ~10,000 probe sets corresponding to expressed sequence tags of the plant host, Medicago truncatula. Protocol: see manufacturer's website

Organism:

Sinorhizobium meliloti; Medicago truncatula

29 Series

275 Samples

Download data: CDF, TXT

(Submitter supplied) We performed transcriptional profiling with chvI gain-of-function (EC220) and reduced-function (EC69) strains. The chvI gain-of-function strain that we used contains a dominant gain-of-function chvI allele in addition to wild-type chvI. We identified genes that, relative to their expression level in the wild type, are both upregulated in the chvI gain-of-function strain and downregulated in the reduced-function strain or vice versa.

Organism:

Medicago truncatula; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL9757

9 Samples

Download data: CEL

(Submitter supplied) In α-proteobacteria, strict regulation of cell cycle progression is necessary for the specific cellular differentiation required for adaptation to diverse environmental niches. The symbiotic lifestyle of Sinorhizobium meliloti requires a drastic cellular differentiation that includes genome amplification. To achieve polyploidy, the S. meliloti cell cycle program must be altered to uncouple DNA replication from cell division. more...

Organism:

Sinorhizobium meliloti 1021; Sinorhizobium meliloti

Type:

Expression profiling by array

Platform:

GPL18182

31 Samples

Download data: TXT

(Submitter supplied) Effect of NCR247 and NCR335 on S. meliloti transcriptosome using Solid4 sequencer

Organism:

Sinorhizobium meliloti 1021

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17212

6 Samples

Download data: XLSX

(Submitter supplied) WT and RNE∆IDR strains were globally profiled for mRNA half-lives using rifampicin treatment followed by RNA-seq. Wild type(WT) was compared to RNE∆IDR strain.

Organism:

Sinorhizobium meliloti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28622

16 Samples

Download data: XLSX