GEO DataSets

(Submitter supplied) We present a novel method of using commercial oligonucleotide expression microarrays for aCGH, enabling DNA copy number measurements and expression profiles to be combined using the same platform. This method yields aCGH data from genomic DNA without complexity reduction at a median resolution of approximately 17,500 base pairs. Due to the well-defined nature of oligonucleotide probes, DNA amplification and deletion can be defined at the level of individual genes and can easily be combined with gene expression data. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) BACKGROUND: Cervical carcinoma develops as a result of multiple genetic alterations. Different studies investigated genomic alterations in cervical cancer mainly by means of metaphase comparative genomic hybridization (mCGH) and microsatellite marker analysis for the detection of loss of heterozygosity (LOH). Currently, high throughput methods such as array comparative genomic hybridization (array CGH), single nucleotide polymorphism array (SNP array) and gene expression arrays are available to study genome-wide alterations. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome variation profiling by genome tiling array; Genome variation profiling by SNP array; SNP genotyping by SNP array

Platforms:

GPL4012 GPL2641 GPL201

40 Samples

Download data: CEL, GPR

(Submitter supplied) Gene amplifications and deletions frequently contribute to tumorigenesis. Characterization of these DNA copy-number changes is important for both the basic understanding of cancer and its diagnosis. Comparative genomic hybridization (CGH) was developed to survey DNA copy-number variations across a whole genome. With CGH, differentially labelled test and reference genomic DNAs are co-hybridized to normal metaphase chromosomes, and fluorescence ratios along the length of chromosomes provide a cytogenetic representation of DNA copy-number variation. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL179

10 Samples

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(Submitter supplied) Data underlying Sander et al., Oncogene 2005, June 20, Comparative genomic hybridization on mouse cDNA microarrays and its application to a murine lymphoma model. Includes both array CGH and expression data. Abstract: Microarray-based formats offer a high-resolution alternative to conventional, chromosome-based comparative genomic hybridization (CGH) methods for assessing DNA copy number alteration (CNA) genome-wide in human cancer. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Genome variation profiling by array

Platforms:

GPL2698 GPL3387

23 Samples

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(Submitter supplied) ULTRA GAPS (Gama Amino Propyl Silane) Protocol: This BAC array platform consisted of 3315 unique BACs spotted in-house on CorningCMT Ultra-Gap slides (Corning, USA) using two replicates printed on each slide. Details of array construction are described elsewhere (Greshock et al., 2004) Greshock J, Naylor TL, Margolin A, Diskin S, Cleaver SH, Futreal PA, deJong PJ, Zhao S, Liebman M, Weber BL. 2004. 1-Mb resolution array-based comparative genomic hybridization using a BAC clone set optimized for cancer gene analysis. Genome Res 14(1):179-87.

Organism:

Homo sapiens

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(Submitter supplied) ULTRA GAPS (Gama Amino Propyl Silane) Protocol: This BAC array platform consisted of 4575 unique BACs spotted in-house on CorningCMT Ultra-Gap slides (Corning, USA) using two replicates printed on each slide. Details of array construction are described elsewhere (Greshock et al., 2004) Greshock J, Naylor TL, Margolin A, Diskin S, Cleaver SH, Futreal PA, deJong PJ, Zhao S, Liebman M, Weber BL. 2004. 1-Mb resolution array-based comparative genomic hybridization using a BAC clone set optimized for cancer gene analysis. Genome Res 14(1):179-87.

Organism:

Homo sapiens

Download data

(Submitter supplied) ULTRA GAPS (Gama Amino Propyl Silane) Protocol: This BAC array platform consisted of 4798 unique BACs spotted in-house on CorningCMT Ultra-Gap slides (Corning, USA) using two replicates printed on each slide. Details of array construction are described elsewhere (Greshock et al., 2004) Greshock J, Naylor TL, Margolin A, Diskin S, Cleaver SH, Futreal PA, deJong PJ, Zhao S, Liebman M, Weber BL. 2004. 1-Mb resolution array-based comparative genomic hybridization using a BAC clone set optimized for cancer gene analysis. Genome Res 14(1):179-87.

Organism:

Homo sapiens

Download data

(Submitter supplied) Ultra GAPS (Gama Amino Propyl Silane) Protocol: This BAC array platform consisted of 5217unique BACs spotted in-house on CorningCMT Ultra-Gap slides (Corning, USA) using two replicates printed on each slide. Details of array construction are described elsewhere (Greshock et al., 2004) Greshock J, Naylor TL, Margolin A, Diskin S, Cleaver SH, Futreal PA, deJong PJ, Zhao S, Liebman M, Weber BL. 2004. 1-Mb resolution array-based comparative genomic hybridization using a BAC clone set optimized for cancer gene analysis. more...

Organism:

Homo sapiens

Download data

(Submitter supplied) ULTRA GAPS (Gama Amino Propyl Silane) Protocol: This BAC array platform consisted of 5215 unique BACs spotted in-house on CorningCMT Ultra-Gap slides (Corning, USA) using two replicates printed on each slide. Details of array construction are described elsewhere (Greshock et al., 2004) Greshock J, Naylor TL, Margolin A, Diskin S, Cleaver SH, Futreal PA, deJong PJ, Zhao S, Liebman M, Weber BL. 2004. more...

Organism:

Homo sapiens

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(Submitter supplied) 60-mers oligonucleotide probes, in-situ synthesis on 1 Protocol: Sure Print Technology-In situ synthesis

Organism:

Staphylococcus aureus

4 Series

30 Samples

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(Submitter supplied) K14Cre; Brca2F/F; p53F/F tumor analysis Keywords: Comparative Genomic Hybridization, aCGH

Organism:

Mus musculus

Type:

Genome variation profiling by genome tiling array

Platform:

GPL2851

4 Samples

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(Submitter supplied) Array-based comparative genomic hybridisation is a high-resolution method for measuring chromosomal copy number changes. Here we present a validated protocol using in-house spotted oligonucleotide libraries for array CGH. This oligo array CGH platform yields reproducible results and is capable of detecting single copy gains, multi-copy amplifications as well as homozygous and heterozygous deletions as small as 100 kb with high resolution. more...

Organism:

Homo sapiens; Mus musculus

Type:

Genome variation profiling by array; Genome variation profiling by genome tiling array

11 related Platforms

36 Samples

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(Submitter supplied) - clones were mapped to the UCSC July 2003 Freeze of the Human Golden Path (NCBI build 34) - oncobacs were mapped using their overgo-primers and are available via www.ensembl.org Protocol: as described in: Carvalho et al (J. Clin. Path.,2004, vol. 57, 644-646). DNA from the 1 Mb resolution Sanger BAC clone set, the OncoBac set, and in house clones of interest, supplemented with clones from the Children’s Hospital Oakland Research Institute (CHORI), amounting to a total of 4678 clones with known chromosomal positions, was isolated according to the CHORI protocol. more...

Organism:

Homo sapiens

2 Series

37 Samples

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(Submitter supplied) hydrophilic polymer containing N-hydroxysuccinimide (NHS) ester reactive groups Protocol: The Human Release 2.0 oligonucleotide library, containing 60-mer oligonucleotides representing 28830 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). The oligonucleotides were dissolved at 10 uM concentration in 50 mM sodium phosphate buffer pH 8.5 and single spotted onto CodeLink slides (Amersham Biosciences), using an OmniGrid 100 microarrayer (Genomic Solutions, Ann Arbor, MI, USA) equipped with SMP3 pins (TeleChem International, Sunnyvale, CA, USA). more...

Organism:

Homo sapiens

1 Series

1 Sample

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(Submitter supplied) - oligonucleotide sequences are available via www.ensembl.org after agreeing to Compugen's conditions - oligonucleotides were mapped by Ensembl to the NCBI35 version of the human genome - all plates were printed once (no replicate spots) Protocol: The Human Release 2.0 oligonucleotide library, containing 60-mer oligonucleotides representing 28830 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys. more...

Organism:

Homo sapiens

1 Series

1 Sample

Download data

(Submitter supplied) - oligonucleotide sequences are available via www.ensembl.org after agreeing to Compugen's conditions - oligonucleotides were mapped by Ensembl to the NCBI35 version of the human genome - all plates were printed once (no replicate spots) Protocol: The Human Release 2.0 oligonucleotide library, containing 60-mer oligonucleotides representing 28830 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). more...

Organism:

Homo sapiens

3 Series

73 Samples

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(Submitter supplied) - oligonucleotide sequences are available via www.ensembl.org after agreeing to Compugen's conditions - oligonucleotides were mapped by Compugen to the July 2003 Freeze of the human genome - all plates were printed once (no replicate spots) Protocol: The Human Release 1.0 oligonucleotide library, containing 18861 60-mer oligonucleotides representing 17260 unique genes as designed by Compugen (San Jose, CA, USA) was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). more...

Organism:

Homo sapiens

2 Series

24 Samples

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(Submitter supplied) - oligonucleotide sequences are available via www.ensembl.org after agreeing to Compugen's conditions - oligonucleotides were mapped by Ensembl to the NCBIm33 version of the mouse genome - all plates were printed once (no replicate spots) Protocol: The Mouse 8K and Mouse 14K extension oligonucleotide libraries, containing 65-mer oligonucleotides representing 21825 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). more...

Organism:

Mus musculus

2 Series

10 Samples

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(Submitter supplied) - clones were mapped to the UCSC July 2003 Freeze of the Human Golden Path (NCBI build 34) - oncobacs were mapped using their overgo-primers and are available via www.ensembl.org Protocol: as described in: Carvalho et al (J. Clin. Path.,2004, vol. 57, 644-646). DNA from the 1 Mb resolution Sanger BAC clone set, the OncoBac set, and in house clones of interest, supplemented with clones from the Children’s Hospital Oakland Research Institute (CHORI), amounting to a total of 5284 clones with known chromosomal positions, was isolated according to the CHORI protocol. more...

Organism:

Homo sapiens

8 Series

160 Samples

Download data

(Submitter supplied) - clones were mapped to the UCSC July 2003 Freeze of the Human Golden Path (NCBI build 34) - oncobacs were mapped using their overgo-primers and are available via www.ensembl.org Protocol: as described in: Carvalho et al (J. Clin. Path.,2004, vol. 57, 644-646). DNA from the 1 Mb resolution Sanger BAC clone set, the OncoBac set, and in house clones of interest, supplemented with clones from the Children’s Hospital Oakland Research Institute (CHORI), amounting to a total of 4678 clones with known chromosomal positions, was isolated according to the CHORI protocol. more...

Organism:

Homo sapiens

4 Series

104 Samples

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