GEO DataSets

(Submitter supplied) Toxoplasma gondii is a ubiquitous protozoan parasite with a complex life cycle containing multiple developmental stages. The parasites have distinct gene expression patterns at different stages to enable stage specific life activities, but the underlying regulatory mechanisms are largely unknown. In this study, we identified a nuclear complex that controls the expression of developmentally regulated genes, especially merozoite specific genes. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26742

8 Samples

Download data: BIGWIG

(Submitter supplied) To investigate the transcriptional effects of TgAP2XII-5 on type II Toxoplasma gondii (ME49) in the tachyzoite and bradyzoite stages. Also, to study the transcriptional role of TgAIP1 on ME49 tachyzoites.

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33297

18 Samples

Download data: TXT, XLS, XLSX

(Submitter supplied) The apicomplexan parasite Toxoplasma gondii can infect humans and almost all warm-blooded animals worldwide, poses significant threat to the public health and of veterinary importance. The acute infection was characterized by fast replication of tachyzoites inside the host cells. During this fast amplification process, the gene expression is highly regulated by series of regulator networks. The G1 phase is usually conserved across species and responsible for the preparation of materials for the next replication cell cycle, but seldom regulators were identified in this stage. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26742

4 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) We performed in vivo CRISPR screens of Toxoplasma targeting hyperLOPIT-unassigened genes during mouse infection.

Organism:

Toxoplasma gondii RH

Type:

Other

Platform:

GPL34114

11 Samples

Download data: TXT, XLSX

(Submitter supplied) We performed in vivo CRISPR screens of Toxoplasma targeting genes with hyperLOPIT-unassigened subcellular localisation during mouse infection.

Organism:

Toxoplasma gondii RH

Type:

Other

Platform:

GPL34114

12 Samples

Download data: TXT, XLSX

(Submitter supplied) Toxoplasmosis is a major health issue worldwide especially for immune-deficient individuals and the offspring of newly infected mothers. It is caused by a unicellular intracellular parasite called Toxoplasma gondii. Although the drugs commonly used to treat toxoplasmosis are efficient, they present serious side effects and adverse events are common. Therefore, there is a need for the discovery of new compounds with potent anti-T. more...

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23377

6 Samples

Download data: XLSX

(Submitter supplied) Toxoplasma gondii is an intracellular parasitic protozoan that poses a significant risk to pregnant women and immunocompromised individuals. T. gondii tachyzoites duplicate rapidly in host cells during acute infection through endodyogeny. This highly regulated division process is accompanied by complex gene regulation networks. TgAP2XII-9 is a cyclical transcription factor, but its specific role in the parasite cell cycle is not fully understood. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26742

4 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) We tested the relevance of an established stem-cell-derived model of trophoblast development to Toxoplasma gondii infection. Human trophoblast stem cells were cultured under conditions suitable for cytotrophoblast cells or for the differentiation into syncytiotrophoblasts, and subsequently infected with T. gondii. RNA-seq data from both mock-treated and infected cells revealed differences between cell types and their respective responses to T. more...

Organism:

Toxoplasma gondii; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL30173 GPL34372

12 Samples

Download data: TXT

(Submitter supplied) Toxoplasma gondii persists in humans by converting from actively replicating acute stage tachyzoites to slow-growing chronic stage bradyzoites. The molecular mechanisms that mediate T. gondii differentiation remain poorly understood. Through a chemical mutagenesis screen, we identified translation initiation factor eIF1.2 as being critical for T. gondii differentiation. The presence of an F97L mutation in eIF1.2 identified in the screen or the complete lack eIF1.2 (∆eIF1.2) markedly impeded bradyzoite cyst formation in culture and in the brains of infected mice. more...

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL23466

24 Samples

Download data: TXT

(Submitter supplied) The protozoan parasite Toxoplasma gondii causes serious opportunistic disease due to its ability to persist in patients as latent tissue cysts. The molecular mechanisms coordinating conversion between proliferative parasites (tachyzoites) and latent cysts (bradyzoites) are not fully understood. We previously showed that phosphorylation of eIF2α accompanies bradyzoite formation, suggesting that this clinically relevant process involves regulation of mRNA translation. more...

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26742

18 Samples

Download data: TABULAR

(Submitter supplied) Commitment to and completion of sexual development are essential for Toxoplasma gondii to produce oocysts in the intestine of the feline host. Understanding of the molecular mechanism responsible for sexual commitment is extremely limited due to the lack of model systems. Here, we show that the transcription factors AP2XI-2 and AP2XII-1 associated with the epigenetic repressors MORC/HDAC3 complex are constitutively expressed in both tachyzoite and bradyzoite stages but not in the merozoite stage. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26742

6 Samples

Download data: BW

(Submitter supplied) The bromdomain protein TgBDP3 is associated with the TFIID transcriptioanl initiation complex in Toxoplasma gondii. To determine the genes that are regulated by TgBDP3, the endogenous tgbdp3 locus was modified to introduce a C-terminal HA epitope tag. ChIP-seq was performed on intracellular, replicating tachyzoites expressing TgBDP3-HA. The protein was enriched in the upstream, promoter region of a subset of actively transcribed parasite genes indicating that TgBDP3 has a role in regulation of transcriptional initiation in Toxoplasma.

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25973

5 Samples

Download data: BED

(Submitter supplied) Ingestion of Toxoplasma gondii results in life-long infection due to its ability to convert from the rapidly disseminating tachyzoite stage to the chronic, encysted bradyzoite stage. The control of mRNA translation has been suggested to play a key role in the signaling required to trigger bradyzoite formation. In eukaryotes, translational control primarily operates at two key points during the assembly of translation initiation factors. more...

Organism:

Toxoplasma gondii

Type:

Other; Expression profiling by high throughput sequencing

Platform:

GPL23466

12 Samples

Download data: TXT

(Submitter supplied) Ingestion of Toxoplasma gondii results in life-long infection due to its ability to convert from the rapidly disseminating tachyzoite stage to the chronic, encysted bradyzoite stage. The control of mRNA translation has been suggested to play a key role in the signaling required to trigger bradyzoite formation. In eukaryotes, translational control primarily operates at two key points during the assembly of translation initiation factors. more...

Organism:

Toxoplasma gondii

Type:

Other

Platform:

GPL23466

12 Samples

Download data: BED, BIGWIG

(Submitter supplied) Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26742

4 Samples

Download data: BW, TXT

(Submitter supplied) Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. more...

Organism:

Toxoplasma gondii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23466

20 Samples

Download data: BW

(Submitter supplied) Reactivation of toxoplasmosis poses a significant health risk to chronically infected people especially those with compromised immune systems. Molecular studies of reactivation have been difficult due to the lack of accurate models of bradyzoite recrudescence, which initiates disease reactivation. Here, we performed single cell RNA-sequencing (scRNA-seq) on parasite populations that form after bradyzoite infection of two host-cell types: primary mouse astrocytes and human foreskin fibroblasts. more...

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26742

6 Samples

Download data: MTX, TSV

(Submitter supplied) Toxoplasma gondii is an intracellular parasite that relies on intricate molecular mechanisms for its proliferation and survival. Here, we investigate the role of TgUAE1 in these processes and shed light on the underlying regulatory network. We demonstrate that TgUAE1 is crucial for maintaining the proliferation of T. gondii and the normal morphology of mitochondria. To elucidate the molecular basis of TgUAE1's essential functions, we conducted transcriptome sequencing to identify the genes regulated by this factor. more...

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26742

8 Samples

Download data: XLSX

(Submitter supplied) There are two experiments included in this particular GEO accession: For the first, freshly excysted sporozoites of T. gondii VEG or H. hammondi Amer1 were put into culture for 2 days, and then either allowed to continue growth in control (pH 7.2, 5% C02, 10% FCS) or high pH, bradyzoite-inducing (pH 8.2, low C02, 1% FCS) conditions for 3 days and then harvested for RNAseq analysis. In the second, T. more...

Organism:

Hammondia hammondi; Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32894 GPL25973

27 Samples

Download data: TXT

(Submitter supplied) Mice were infected with up to 250,000 tachyzoites of T. gondii VEG strain parasites genetically engineered to be deficient in either the ROCY1 gene (TGVEG_311100) or the BFD1 gene (TGVEG_200385). Mice were infected intraperitoneally and monitored using in vivo bioluminescence imaging. Mice were sacrificed at either 4 weeks or 9 weeks post infection as indicated, and RNA was isolated from their brains and transcript abundance was determined using RNAseq.

Organism:

Toxoplasma gondii; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32893

19 Samples

Download data: XLSX