Rossi_et_al_2005_sample_processing; Long-term hematopoietic stem cells (Lineage negative, c-kit positive, Sca1 positive, Flk2 negative and CD34 negative ie. KLSflk2-CD34-) from old mice (22-23 months). Cells were double FACS sorted for purity with the second sort done directly into Trizol (Gibco BRL, Gaithersburg, MD). mRNA from 22,000-25,000 cells were twice amplified using Arcturus RiboAmp RNA amplification kit (Arcturus, Mountain View, CA). Amplified material (cRNA) was biotin-labeled using Affymetrix IVT labelling kit (Affymetrix, Santa Clara, CA) and hybridized to Affymetrix Mouse Genome 430 2.0 arrays.; Protocol Type = AffyExp; Performer: Rebecca,,Sonu
Label
biotin
Description
Mouse HSC transcript levels from Old Cohort
Data processing
VALUE is Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm)
A measurement indicating if the transcript was detected (Present), not detected (Absent) or marginally detected (Marginal). Can be one of "A", "M", "P" or "No Call".; Type: string_datatype; Scale: unscaled
DETECTION P-VALUE
See Detection p-value; Type: float; Scale: linear_scale
STAT_PAIRS_USED
The number of probe pairs in the probe set used in the Detection call.; Type: integer; Scale: linear_scale
VALUE
A measure of the relative abundance of a transcript.; Type: float; Scale: log_base_2
SIG_STD_ERR
Standard error of the signal.; Type: float; Scale: log_base_2
