|
| Status |
Public on Mar 15, 2006 |
| Title |
Cell intrinsic alterations underlie hematopoietic stem cell aging |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Loss of immune function and an increased incidence of myeloid leukemia are two of the most clinically significant consequences of aging of the hematopoietic system. To better understand the mechanisms underlying hematopoietic aging, we evaluated the cell intrinsic functional and molecular properties of highly purified long-term hematopoietic stem cells (LT-HSCs) from young and old mice. We found that LT-HSC aging was accompanied by cell autonomous changes, including increased stem cell self-renewal, differential capacity to generate committed myeloid and lymphoid progenitors, and diminished lymphoid potential. Expression profiling revealed that LT-HSC aging was accompanied by the systemic down-regulation of genes mediating lymphoid specification and function and up-regulation of genes involved in specifying myeloid fate and function. Moreover, LT-HSCs from old mice expressed elevated levels of many genes involved in leukemic transformation. These data support a model in which age-dependent alterations in gene expression at the stem cell level presage downstream developmental potential and thereby contribute to age-dependent immune decline, and perhaps also to the increased incidence of leukemia in the elderly.
Groups of assays that are related as part of a time series.
Keywords: time_series_design
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| |
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| Overall design |
5 old mice and 3 young mice
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| |
|
| Contributor(s) |
Sonu R |
| Citation(s) |
15967997 |
| |
| Submission date |
Mar 03, 2006 |
| Last update date |
Feb 11, 2019 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
[email protected]
|
| Phone |
650-498-6012
|
| URL |
http://genome-www5.stanford.edu/
|
| Department |
Stanford University, School of Medicine
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA94759 |
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