|
| Status |
Public on Apr 07, 2015 |
| Title |
S. cerevisiae BY4741_dodecane vs control_24hrs_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
n-dodecane, 24hrs
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4741
|
| Treatment protocol |
Multiple conditons: yeast cells from overnight cell culture were diluted to OD600=0.2 in medium (1.6g/L yeast nitrogen base, 2% D-glucose,0.1g/L uracine, 0.2g/L, leucine) added with 2% n-alkanes (n-nonane, n-decane, n-undecane, n-dodecane, respectively), and were incubated at 225rpm and 28 Celsius degree in 50mL falcon tubes, for 24hrs or 48hrs. Cells added with sterile H2O instead of n-alkanes were used as control.
|
| Growth protocol |
In YPD yeast cells were grown for overnight at 225rpm and 30 Celsius degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Mini kit (Qiagen, Inc., Valencia, CA) according to the manufacturer’s protocol
|
| Label |
Cy5
|
| Label protocol |
Approximately 200ng of total RNA was processed to produce Cy5 or Cy3-labeled cRNA targets by using Low Input Quick Amp Labeling Kit (Agilent Technologies, USA) according to the manufacture's protocol
|
| |
|
| Channel 2 |
| Source name |
non-treatment (control), 24hrs
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BY4741
|
| Treatment protocol |
Multiple conditons: yeast cells from overnight cell culture were diluted to OD600=0.2 in medium (1.6g/L yeast nitrogen base, 2% D-glucose,0.1g/L uracine, 0.2g/L, leucine) added with 2% n-alkanes (n-nonane, n-decane, n-undecane, n-dodecane, respectively), and were incubated at 225rpm and 28 Celsius degree in 50mL falcon tubes, for 24hrs or 48hrs. Cells added with sterile H2O instead of n-alkanes were used as control.
|
| Growth protocol |
In YPD yeast cells were grown for overnight at 225rpm and 30 Celsius degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Mini kit (Qiagen, Inc., Valencia, CA) according to the manufacturer’s protocol
|
| Label |
Cy3
|
| Label protocol |
Approximately 200ng of total RNA was processed to produce Cy5 or Cy3-labeled cRNA targets by using Low Input Quick Amp Labeling Kit (Agilent Technologies, USA) according to the manufacture's protocol
|
| |
|
| |
| Hybridization protocol |
Standard Agilent procedures
|
| Scan protocol |
Scanned on Agilent Scanner G2505B US45103124, and images were quantified using Agilent Feature Extraction Software v. 10.7
|
| Data processing |
Agilent Feature Extraction Software v. 10.7 was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 12, 2012 |
| Last update date |
Apr 07, 2015 |
| Contact name |
Hua Ling |
| Organization name |
Nanyang Technological University
|
| Department |
Department of Chemical and Biomolecular Engineering, SCBE
|
| Lab |
Prof. Matthew Wook Chang
|
| Street address |
62 Nanyang Drive
|
| City |
Singapore |
| ZIP/Postal code |
637459 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL9825 |
| Series (1) |
| GSE38653 |
Saccharomyces cerevisiae BY4741 cells treated with n-alkanes |
|
