|
| Status |
Public on Jan 01, 2014 |
| Title |
CAG-TKO_3 |
| Sample type |
RNA |
| |
|
| Source name |
Cortical explant Rb-TKO cells labeled by pCAG-Cre
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: cortical explant cell
genotype/variation: Rb-TKO
labeled by: pCAG-Cre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10 ng of the total RNA was purified by RNeasy Micro (QIAGEN)
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified following One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling), ver 6.0.
|
| |
|
| Hybridization protocol |
The amplified cRNA (1.5 µg) was hybridized on Agilent Whole mouse Genome Array (4 × 44K) ver 2.0 for 17 hours.
|
| Scan protocol |
The Chips were scanned on a Agilent DNA Microarray Scanner using Feature Extraction software ver 10.7.1.1 (Agilent).
|
| Description |
Cortical explant Rb-TKO cells labeled by pCAG-Cre
|
| Data processing |
Normalized by pergentile shift (75%) and baseline to median of all samples; GeneSpring software ver 11.5.
Entities were filtered based on their signal intensity value (40-100%), and then the standard deviation (< 0.1). Filtered data linked as supplementary file on Series record.
|
| |
|
| Submission date |
Apr 25, 2012 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Itsuki Ajioka |
| Organization name |
Tokyo Medical and Dental University
|
| Department |
Center for Brain Integration Research
|
| Street address |
1-5-45 Yushima, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE37577 |
Gene expression profile of cerebral cortical cells after Rb family inactivation in progenitors and neurons |
|
