|
| Status |
Public on Oct 10, 2013 |
| Title |
TET2 Mutant mRNA 6 |
| Sample type |
RNA |
| |
|
| Source name |
lymphoma biopsies
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: TET2 Mutant
tissue: lymphoma biopsy
|
| Growth protocol |
Patient Samples
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from fresh frozen lymphoma biopsies using RNeasy mini kit (Qiagen Valenica, CA)
|
| Label |
biotin
|
| Label protocol |
Starting from 5 µg total RNA, labeled cRNA was prepared using the Roche Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16hr on Affymetrix HuGene-1_0-st [transcript (gene version)] as recommended by manufacturer
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression data from TET2 mutant patient sample
|
| Data processing |
The data was analyzed with Gene Spring GX 12
|
| |
|
| Submission date |
Apr 17, 2012 |
| Last update date |
Oct 10, 2013 |
| Contact name |
Vasu Punj |
| E-mail(s) |
[email protected]
|
| Organization name |
USC
|
| Street address |
Biggy Street
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE37363 |
TET2 loss-of-function mutations associate with a DNA hypermethylation signature in diffuse large B-cell lymphoma (mRNA) |
| GSE37365 |
TET2 loss-of-function mutations associate with a DNA hypermethylation signature in diffuse large B-cell lymphoma |
|
