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Status
Public on Dec 09, 2005
Title
3D3, chip MG_U74Cv2
Sample type
RNA
Source name
Mesenchymal cells, Limb bud
Organism
Mus musculus
Characteristics
Strain: CD-1 Defining markers: Sox9, type II collagen
Treatment protocol
Assays to confirm viability: Cells were allowed to differentiate <i>in vitro</i> into chondrocytes
Extracted molecule
total RNA
Extraction protocol
RNA prep method: RNeasy Cell purification method: Cells were isolated from the subridge region of the developing limb Contaminating cell type: Fibroblasts Method for estimating purity: Morphology of cultures 3 days post harvest
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/ ). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/ ) by Dr. T. Michael Underhill ([email protected] ; 1400 Western Road) for analysis. Stembase Experiment ID: E30 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30 SCGP Sample ID: S34 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=30#SAMPLE_32 Short description: Chondroblasts harvested at 3 days. Estimated purity: 90-95% Num cells for RNA prep: 6x10e6
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
Submission date
Dec 07, 2005
Last update date
Jan 19, 2006
Organization
Ottawa Hospital Research Institute
Phone
(613) 737-8899 -73255
Department
Cellular and Molecular Medicine
Lab
Ottawa Bioinformatics Core Facility
Street address
501 Smyth Rd.
City
Ottawa
State/province
ON
ZIP/Postal code
K1H 8L6
Country
Canada
Platform ID
GPL83
Series (1)
GSE3770
Temporal Analysis of Differentiating Limb Bud Mesenchymal Cell Gene Expression
Data table header descriptions
ID_REF
VALUE
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-MurIL2_at
249.482
A
0.941556
AFFX-MurIL10_at
839.625
A
0.5
AFFX-MurIL4_at
313.391
A
0.783476
AFFX-MurFAS_at
1986.83
P
0.0125468
AFFX-BioB-5_at
13684
P
0.00110197
AFFX-BioB-M_at
21668.3
P
5.16732e-05
AFFX-BioB-3_at
14446.1
P
9.4506e-05
AFFX-BioC-5_at
34681.3
P
0.000146581
AFFX-BioC-3_at
27439.9
P
5.16732e-05
AFFX-BioDn-5_at
36646.2
P
4.42873e-05
AFFX-BioDn-3_at
233790
P
8.14279e-05
AFFX-CreX-5_at
447193
P
4.42873e-05
AFFX-CreX-3_at
484038
P
4.42873e-05
AFFX-BioB-5_st
2481.78
A
0.147939
AFFX-BioB-M_st
2572.46
A
0.48511
AFFX-BioB-3_st
1461.35
A
0.5
AFFX-BioC-5_st
541.286
A
0.794268
AFFX-BioC-3_st
263.625
A
0.659339
AFFX-BioDn-5_st
4514.27
A
0.102165
AFFX-BioDn-3_st
4904.26
A
0.0726999
Total number of rows: 11934 Table truncated, full table size 346 Kbytes .Supplementary file
Size
Download
File type/resource
GSM86527.CEL.gz
2.7 Mb
(ftp) (http)
CEL
GSM86527.EXP.gz
522 b
(ftp) (http)
EXP