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Status
Public on Dec 01, 2005
Title
untreated 3 h replicate 3
Sample type
RNA
Source name
suspension cell culture, untreated, 3 h
Organism
Arabidopsis thaliana Characteristics
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
Biomaterial provider
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Pia Sappl
Extracted molecule
total RNA
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type Arabidopsis suspension cell culture, pale green in colour
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Dec 01, 2005
Contact name
Rachel Clifton
E-mail(s)
[email protected] Organization name
Plant Energy Biology, ARC Centre of Excellence
Department
Biochemistry, UWA
Lab
Plant Molecular Biology Group
Street address
4th Floor CMS Building M310, University of Western Australia
City
Crawley
State/province
Western Australia
ZIP/Postal code
6009
Country
Australia
Platform ID
GPL198 Series (1)
GSE3709 Cell culture treatment study
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
316.6
P
0.000258
AFFX-BioB-M_at
372.3
P
0.000052
AFFX-BioB-3_at
301.7
P
0.000052
AFFX-BioC-5_at
705.2
P
0.00006
AFFX-BioC-3_at
541.4
P
0.00006
AFFX-BioDn-5_at
792.3
P
0.000044
AFFX-BioDn-3_at
3435.7
P
0.000044
AFFX-CreX-5_at
5195.1
P
0.000044
AFFX-CreX-3_at
8434.5
P
0.000044
AFFX-DapX-5_at
376.8
P
0.00007
AFFX-DapX-M_at
679.5
P
0.000052
AFFX-DapX-3_at
1032.7
P
0.000044
AFFX-LysX-5_at
43.4
P
0.000581
AFFX-LysX-M_at
98.9
P
0.005565
AFFX-LysX-3_at
113.4
P
0.000509
AFFX-PheX-5_at
102.8
P
0.000754
AFFX-PheX-M_at
117
P
0.011384
AFFX-PheX-3_at
151.4
P
0.000258
AFFX-ThrX-5_at
95.6
P
0.000297
AFFX-ThrX-M_at
186.5
P
0.000581
22810 586 Kbytes .Supplementary data files not provided
