|
Status |
Public on Nov 13, 2024 |
Title |
optic tectum batch 4, scRNAseq |
Sample type |
SRA |
|
|
Source name |
optic tectum
|
Organism |
Danio rerio |
Characteristics |
tissue: optic tectum age: 7 day post fertilization cell type: all cells genotype: wild type treatment: no treatment
|
Extracted molecule |
total RNA |
Extraction protocol |
The optic tectum and torus longitudinalis of 6 and 7 dpf larvae were carefully dissected under a stereoscope. Cell dissociation was performed using the Papain Dissociation System (Worthington Biochemical Corporation). libraries according to the manufacturer’s instructions (Chromium Single Cell 3′ Reagent Kit v3, 10x Genomics). single-cell 3′ barcoded cDNA
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
10x Genomics dr_7dpf_tectum4_S1
|
Data processing |
Demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v7.1.0 Assembly: GRCz11 genome assembly (Ensembl release 98) Supplementary files format and content: Tab-separated values files and matrix files
|
|
|
Submission date |
Jun 06, 2024 |
Last update date |
Nov 13, 2024 |
Contact name |
Inbal Shainer |
Organization name |
Max Planck Institute for Biological Intelligence
|
Street address |
Am Klopferspitz 18
|
City |
Martinsried |
ZIP/Postal code |
82152 |
Country |
Germany |
|
|
Platform ID |
GPL20828 |
Series (1) |
GSE269232 |
Positional information drives distinct traits in transcriptomically identified neuronal types |
|
Relations |
BioSample |
SAMN41708634 |
SRA |
SRX24823815 |