|
| Status |
Public on Aug 01, 2012 |
| Title |
heart_control_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
gender: male
sample type: positive for Cre recombinase, but negative for the floxed IGF-1R
|
| Treatment protocol |
At the age of 8 month the animals were treated with 4OH-Tamoxifen (500 µg dissolved in 100 µl peanut oil) on 10 consecutive days to excise Exon 3 of the IGF-1R. Six weeks after the treatment hearts were removed for further analysis.
|
| Growth protocol |
Mice were bred at the Tierversuchsanlage of the Heinrich-Heine-Universitaet, Duesseldorf, Germany. Animal experiments were performed in accordance with the national guidelines on animal care and approved by the Bezirksregierung Duesseldorf. Animals received standard rodent chow diet and tap water ad libitum and were kept on a dark/light cycle of 12/12 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was isolated using the Trizol reagent (Invitrogen) following manufactures instructions. After ispropanol precipitation a clean up step using RNeasy Mini columns (Qiagen) were performed.
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA were reverse transcribed, linear amplified and labeled using the One Color Low Input Qick Amp Labeling Kit (Agilent).
|
| |
|
| Hybridization protocol |
The fragmentated Cy3 labeled cRNA were hybridised on Agilent Mouse GE 4x44K v2 arrays in Hi-RPM Hybridization Buffer (Agilent) at 65°Cand 65 rpm for 17h.
|
| Scan protocol |
Scannning of the arrays were performed by the Agilent High Resolution Scanner G2505C.
|
| Data processing |
The fluorescence intensities were calculated with the Feature Extraction Software Version 10.7.3.1. For further data analysis the FE files were imported to GeneSpring GX Version 11.
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Antje Lindecke |
| E-mail(s) |
[email protected]
|
| Phone |
49-211-8114367
|
| Organization name |
Universitaet Duesseldorf
|
| Department |
BMFZ
|
| Lab |
Microarray
|
| Street address |
Universitaetsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE32936 |
IGF1-receptor signalling attenuates the age-related decline of diastolic cardiac function |
|
