|
| Status |
Public on Jun 29, 2012 |
| Title |
hESCs control for RB7LP, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human embryonic stem cells
cell line: H9 line
age: p58
|
| Treatment protocol |
hESCs were plated in duplicate overnight before doxycycline was added to the media to half of the wells to induce expression of either RB7LP or T121. Three days after doxycycline treatment began, cells were trypsinized, washed, pelleted and snap frozen before RNA extraction.
|
| Growth protocol |
hESCs were maintained on irradiated feeders as described previously ((E.Chiao et al. Stem Cells 26, 2032 (Aug, 2008).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express kit protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, labelled cRNA were hyb using GeneChip Hybridization Oven 640 for 16h. GeneChips were washed and stained in the Genechip Fluidics Station 450
|
| Scan protocol |
The arrays were scanned by Affymetrix GeneChip Scanner 3000 7G and the software used was Affymetrix GeneChip Command Console
|
| Description |
H9 hESCs control for RB7LP
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Affymetrix default analysis settings and quantile as normalization method.
|
| |
|
| Submission date |
Jun 07, 2011 |
| Last update date |
Jun 29, 2012 |
| Contact name |
Julien Sage |
| E-mail(s) |
[email protected]
|
| Phone |
(650) 723-5113
|
| Organization name |
Stanford University
|
| Department |
Pediatrics
|
| Lab |
Sage
|
| Street address |
265 Campus Drive, SIM1 G2078
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5457 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE29783 |
Expression data from hESCs expressing RB7LP or T121 |
|
