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| Status |
Public on Jun 29, 2012 |
| Title |
Expression data from hESCs expressing RB7LP or T121 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used microarray analysis to study the expression differences between controls and hESCs expressing RB7LP for 3 days, and controls and hESCs expressing T121 for 3 days. RB7LP is the large pocket fragment of the retinoblastoma protein (RB) fused to GFP,
in which seven phosphorylation sites for Cdk have been mutated (Angus, SP et al. Mol Cell Biol 23, 8172 (Nov, 2003). T121 is a truncated form of the SV40 Large T (LT) viral oncoprotein that inactivates the function of the three members of the retinoblastoma (RB) protein family (RB, p107, p130).
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| Overall design |
We plated hESCs that had been infected with inducible lentiviruses to express either RB7LP or T121. The day after plating, one well from each virus was treated with doxycyline to induce expression of either RB7LP or T121 and after 3 days were harvested for microrarray analysis.
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| Contributor(s) |
Conklin JF, Sage J |
| Citation(s) |
23212373 |
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| Submission date |
Jun 07, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Julien Sage |
| E-mail(s) |
[email protected]
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| Phone |
(650) 723-5113
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| Organization name |
Stanford University
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| Department |
Pediatrics
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| Lab |
Sage
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| Street address |
265 Campus Drive, SIM1 G2078
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5457 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| GSM738195 |
hESCs control for RB7LP, biological rep 1 |
| GSM738196 |
hESCs control for RB7LP, biological rep 2 |
| GSM738197 |
hESCs expressing RB7LP for 3d, biological rep 1 |
| GSM738198 |
hESCs expressing RB7LP for 3d, biological rep 2 |
| GSM738199 |
hESCs control for T121, biological rep 1 |
| GSM738200 |
hESCs control for T121, biological rep 2 |
| GSM738201 |
hESCs control for T121, biological rep 3 |
| GSM738202 |
hESCs expressing T121, biological rep 1 |
| GSM738203 |
hESCs expressing T121, biological rep 2 |
| GSM738204 |
hESCs expressing T121, biological rep 3 |
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| Relations |
| BioProject |
PRJNA141057 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29783_RAW.tar |
47.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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