|
| Status |
Public on Dec 28, 2023 |
| Title |
02_mRNAseq_MWT_HSC_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Hematopoetic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Born Marrow
cell type: Hematopoetic stem cells
genotype: WT
treatment: None
age: Mid-age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified with TRIzol TM Reagent (Invitrogen, 15596026) and Direct-zol RNA microprep (Zymo R2060)
RNA-seq libraries were constructed using SMARTer Standard mRNA-seq kit v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
cutadapt trimming
STAR alignment (RNA-seq)
featureCount, gene-level extraction (RNA-seq)
Assembly: mm10
Supplementary files format and content: text file
|
| |
|
| Submission date |
Feb 28, 2023 |
| Last update date |
Dec 28, 2023 |
| Contact name |
Bongsoo Park |
| E-mail(s) |
[email protected]
|
| Phone |
1-410-558-8510
|
| Organization name |
National Institute on Aging
|
| Department |
Translational Gerontology Branch
|
| Lab |
Epigenetics and Stem Cell Aging
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE226334 |
TTP overexpression inhibits reconstitution potential of HSC but generates a dominant mitigating effect in collagen antibody-induced arthritis mice models |
|
| Relations |
| BioSample |
SAMN33546468 |
| SRA |
SRX19531314 |
