NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE226334 Query DataSets for GSE226334
Status Public on Dec 28, 2023
Title TTP overexpression inhibits reconstitution potential of HSC but generates a dominant mitigating effect in collagen antibody-induced arthritis mice models
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tristetraprolin (TTP), encoded by Zfp36 in mice, is one of the best characterized tandem zinc-finger mRNA binding protein involved in mRNA deadenylation and decay. TTPΔARE mice lack an AU-rich motif in the 3’ untranslated regions of TTP mRNA leading to increased TTP mRNA stability and more TTP protein, resulting in elevated mRNA decay rates of TTP targets. We examined the effect of TTP overexpression on the hematopoietic system and found alterations in red blood cell and white blood cell frequencies, with loss of platelets and B220 cells and gains of eosinophils and T-cells. TTPΔARE mice also have skewed primitive populations in the bone marrow with increases in myeloid-biased HSCs, but loss of granulocyte/macrophage biased MPP3 in both young and mid-aged mice. These populations changes were associated with cell-specific transcriptional alterations, but surprisingly consistent upregulation of TNF and TGFB signaling pathways in both progenitor populations. HSCs with overexpression of TTP had decreased reconstitution potential but generate hematopoietic environments that mitigate the inflammatory response induced by collagen antibody-induced arthritis (CAIA) model- even when TTP overexpressing cells are present at low frequencies. We present an overall analysis of elevated TTP expression in the early hematopoietic compartments which drives reduced overall reconstitution potential of the HSCs but leads to a dominant repression of inflammation induced in a rheumatoid arthritis model.
 
Overall design Comparative gene expression profiling analysis of RNA-seq data for hematopetic stem cells (HSCs) and TPPdeltaARE HSCs
 
Contributor(s) Tanaka-Yano M, Zong L, Park B, Beerman I
Citation(s) 37535204
Submission date Feb 28, 2023
Last update date Mar 28, 2024
Contact name Bongsoo Park
E-mail(s) [email protected]
Phone 1-410-558-8510
Organization name National Institute on Aging
Department Translational Gerontology Branch
Lab Epigenetics and Stem Cell Aging
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (24)
GSM7073450 01_mRNAseq_MWT_HSC_rep1
GSM7073451 02_mRNAseq_MWT_HSC_rep2
GSM7073452 03_mRNAseq_MWT_HSC_rep3
Relations
BioProject PRJNA939718

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE226334_RAW.tar 6.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap