|
| Status |
Public on Feb 09, 2011 |
| Title |
Infected leaves_14dpi_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
infected oilseed rape leaves 14 days post infection
|
| Organisms |
Brassica napus; Plenodomus lingam |
| Characteristics |
strain: v23.1.3
tissue: derived from infected Brassica napus leaves
time point: 14 days post infection
|
| Growth protocol |
Mycelium was grown during one week in Fries liquid medium. Oilseed rape leaves were infected with Leptosphaeria maculans (isolate v23.1.3) spores and harvested 7 and 14 days post inoculation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was carried out using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. Total RNA was treated with DNase I RNase-Free (New England Biolabs).
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company.
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company.
|
| Scan protocol |
Scanning by NimbleGen Company.
|
| Description |
42540702_532
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Feb 08, 2011 |
| Last update date |
Feb 09, 2011 |
| Contact name |
Isabelle Fudal |
| E-mail(s) |
[email protected]
|
| Phone |
33-130814590
|
| Organization name |
INRA
|
| Department |
UR1290
|
| Lab |
BIOGER-CPP
|
| Street address |
avenue Lucien Bretignieres
|
| City |
Thiverval-Grignon |
| ZIP/Postal code |
78850 |
| Country |
France |
| |
|
| Platform ID |
GPL13139 |
| Series (1) |
| GSE27152 |
Leptosphaeria maculans gene expression during in vitro growth and primary oilseed rape leaf infection |
|
