|
| Status |
Public on May 18, 2023 |
| Title |
RA_Patient_4_TCR_R1 |
| Sample type |
SRA |
| |
|
| Source name |
synovium
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: synovium
cell type: helper T cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Synovium samples from four RA patients were obtained. Single-cell suspensions were prepared as previously described (PMID: 29996944). CD4+ T cells were magnetically sorted by MojoSort Mouse CD4 T Cell Isolation Kit, and susequently CD3+ CD4+ T cells were sorted by a FACS sorter.
Sorted cells were immediately subjected to 10X Chromium controler to prepare libraries. Libraries were prepared using Chromium Next GEM Single Cell 5'Kit v2 and Single-Cell Human TCR Amplificication Kit according to the protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Description |
patient 4
DK21229_04_VDJ_S4
|
| Data processing |
Basecalls and mapping performed using Cell Ranger v6.
Assembly: GRCh38
|
| |
|
| Submission date |
Oct 20, 2022 |
| Last update date |
May 18, 2023 |
| Contact name |
Shigeru Tanaka |
| E-mail(s) |
[email protected]
|
| Organization name |
Chiba University
|
| Department |
Allergy and Clinical Immunology
|
| Street address |
1-8-1 Inohana
|
| City |
Chiba |
| ZIP/Postal code |
2608670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL28038 |
| Series (1) |
| GSE216245 |
Single-cell immunoprofiling of synovial helper T cell of rheumatoid arthritis patients. |
|
| Relations |
| BioSample |
SAMN31393523 |
| SRA |
SRX17978277 |
