|
| Status |
Public on Nov 29, 2011 |
| Title |
LB vs 1d in vivo#2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
log phase LB (OD600 = 1.0)
|
| Organism |
Proteus mirabilis |
| Characteristics |
strain: HI4320
growth condition: log phase LB (OD600 = 1.0)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was stabilized using RNA Protect (Qiagen) and isolated using the RNeasy kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
cDNA was generated and labeled with CyDye according to TIGR SOP#M007
|
| |
|
| Channel 2 |
| Source name |
1d in vivo
|
| Organism |
Proteus mirabilis |
| Characteristics |
strain: HI4320
growth condition: 1d in vivo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was stabilized using RNA Protect (Qiagen) and isolated using the RNeasy kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
cDNA was generated and labeled with CyDye according to TIGR SOP#M007
|
| |
|
| |
| Hybridization protocol |
TIGR SOP#M008
|
| Scan protocol |
Slides were scanned using a ScanArray Express microarray scanner (Perkin Elmer) at 10 um resolution and quantified using ScanArray Express software.
|
| Description |
Biological replicate 2 of 3
|
| Data processing |
Total normalization using MIDAS (TIGR) and analysis using MeV (TIGR).
|
| |
|
| Submission date |
Dec 09, 2010 |
| Last update date |
Nov 29, 2011 |
| Contact name |
Melanie M Pearson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan Medical School
|
| Department |
Microbiology and Immunology
|
| Lab |
Melanie Pearson
|
| Street address |
1150 W Medical Center Dr
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-0620 |
| Country |
USA |
| |
|
| Platform ID |
GPL9078 |
| Series (1) |
| GSE25977 |
Transcriptome of Proteus mirabilis in the murine urinary tract |
|
