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| Status |
Public on Nov 29, 2011 |
| Title |
Transcriptome of Proteus mirabilis in the murine urinary tract |
| Platform organism |
Proteus mirabilis HI4320 |
| Sample organism |
Proteus mirabilis |
| Experiment type |
Expression profiling by array
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| Summary |
The enteric bacterium Proteus mirabilis is a common cause of complicated urinary tract infections. In the study, microrarrays were used to analyze P. mirabilis gene expression in vivo from experimentally infected mice. Urine was collected at 1, 3, and 7d postinfection, and RNA was isolated from bacteria in the urine for transcriptional analysis. Across 9 microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. Genes upregulated in vivo encoded MR/P fimbriae, urease, iron uptake systems, amino acid and peptide transporters, pyruvate metabolism, and portions of the TCA cycle. Flagella were downregulated. Ammonia assimilation gene glnA (glutamine synthetase) was repressed in vivo while gdhA (glutamate dehydrogenase) was upregulated in vivo. Contrary to our expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene expression. A gdhA mutant was growth-deficient in minimal medium with citrate as the sole carbon source, and loss of gdhA resulted in a significant fitness defect in the mouse model of urinary tract infection. Unlike Escherichia coli, which represses gdhA and upregulates glnA in vivo and cannot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor carbon-nitrogen balance, and this ability contributes to the pathogenic potential of P. mirabilis in the urinary tract.
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| Overall design |
Voided urine from female CBA/J mice infected with Proteus mirabilis was collected and pooled in RNA stabilizing reagent (RNAprotect). Urine was collected at 1, 3, and 7 d postinfection. RNA was isolated from urine and log-phase LB cultures, converted to cDNA, and labeled with CyDye. Three arrays were completed per time point (9 arrays total). Slides were scanned with a ScanArray Express microarray scanner (Perkin Elmer) at 10 μm resolution and quantified using ScanArray Express software. Resulting data were normalized by total intensity and median spot intensities were identified using MIDAS (v. 2.22) software.
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| Contributor(s) |
Pearson MM, Mobley HL |
| Citation(s) |
21505083 |
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| Submission date |
Dec 09, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Melanie M Pearson |
| E-mail(s) |
[email protected]
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| Organization name |
University of Michigan Medical School
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| Department |
Microbiology and Immunology
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| Lab |
Melanie Pearson
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| Street address |
1150 W Medical Center Dr
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-0620 |
| Country |
USA |
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| Platforms (1) |
| GPL9078 |
UMich_Proteus mirabilis_v1.0 3.7k array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA135519 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25977_RAW.tar |
520.0 Kb |
(http)(custom) |
TAR (of MEV) |
| Processed data included within Sample table |
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