|
| Status |
Public on Mar 30, 2024 |
| Title |
Hp_MPA_2 |
| Sample type |
RNA |
| |
|
| Source name |
single seizure
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: bilateral hippocampus
Sex: male
group: mpa
|
| Treatment protocol |
tfs-mpa rats received tfs (300Hz, 50mA) for 5 minutes, 5 minutes after they received an injection with 3-mercaptopropionic acid (37.5 mg/kg, i.p.). Four hours after the sample was collected. Rats in the mpa groups only received the dose of the proconvulsant. Rats in the tfs group only received the stimulation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
rats were sacrificied by decapitation and the brain was dissected immediately after in cold conditions. All samples were stores at -70°C until use.
|
| Label |
biotin
|
| Label protocol |
Label was performed according to the manufacturer's recommendations in the GeneChip WT Plus Reagent Kit.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufacturer's recommendations in the GeneChip WT Plus Reagent Kit.
|
| Scan protocol |
The chips were scanned with a GeneChip Scanner Affymetrix 3000 7G
|
| Data processing |
Data normalization and statistical analyzes were performed in R (Bioconductor). Two main packages were used: affy and limma. Raw microarray data were normalized using the robust multichip average (RMA) normalization method. For differential gene expression, general linear models and empirical Bayes statistics were used. An absolute logarithmic fold change in expression greater than 1 and an unadjusted P-value lower than 0.05 were thresholds used for gene selection.
|
| |
|
| Submission date |
Mar 22, 2022 |
| Last update date |
Mar 30, 2024 |
| Contact name |
Iris Angélica Feria |
| E-mail(s) |
[email protected]
|
| Phone |
5533320788
|
| Organization name |
Instituto Mexicano del Seguro Social
|
| Department |
UIMEN
|
| Lab |
Biol Mol
|
| Street address |
Cuauhtemoc 330, Doctores
|
| City |
CDMX |
| State/province |
CDMX |
| ZIP/Postal code |
06720 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL23040 |
| Series (1) |
| GSE199183 |
Cortical and hippocampal gene expression after transcranial focal stimulation and a single seizure in rats |
|
