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Sample GSM596054 Query DataSets for GSM596054
Status Public on Dec 06, 2010
Title Gene expression_sex_1328UT
Sample type RNA
 
Source name Biceps Brachii
Organism Homo sapiens
Characteristics gender: female
age: young
condition: resting
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from frozen tissue with a polytron homogenizer (Brinkman, Westbury, NY) and Trizol reagent (Invitrogen, Rockville, MD) and purified with an RNAse kit (Qiagen, Santa Clara, CA).
Label Biotin
Label protocol Total RNA was used as a template for double-stranded cDNA synthesis (Superscript Double-Stranded cDNA synthesis kit, Invitrogen, Carlsbad, CA). Biotin-labelled cRNA was synthesized by using Enzo Bioarray High Yield RNA transcription Labeling Kit (Affymetrix).
 
Hybridization protocol Hybridized to Affymetrix Human Genome U133 Plus 2.0 arrays (Santa Clara, CA) according to the manufacturer’s instructions.
Scan protocol The intensity of bound dye was measured with an argon laser confocal scanner (GeneArray Scanner, Agilent).
Data processing The probe arrays were scanned twice and the stored images were analyzed using the Expression Console (Affymetrix, Santa Clara, CA). RMA data was derived by using Bioconductor.
 
Submission date Sep 20, 2010
Last update date Dec 06, 2010
Contact name GORDON PAUL
E-mail(s) [email protected]
Organization name University of Michigan
Street address 325 E Eisenhower
City Ann Arbor
State/province MI
ZIP/Postal code 48108
Country USA
 
Platform ID GPL570
Series (1)
GSE24235 Skeletal muscle gene expression in response to resistance exercise: sex specific regulation

Data table header descriptions
ID_REF
VALUE RMA data

Data table
ID_REF VALUE
1007_s_at 8.054704666
1053_at 4.747791686
117_at 3.524663192
121_at 6.879585679
1255_g_at 2.85433223
1294_at 4.616840024
1316_at 4.439242354
1320_at 3.914933565
1405_i_at 2.994000473
1431_at 2.941122617
1438_at 4.786534392
1487_at 8.103801169
1494_f_at 4.845900138
1552256_a_at 4.940750302
1552257_a_at 5.427276684
1552258_at 3.33929127
1552261_at 3.662862472
1552263_at 2.881587622
1552264_a_at 6.320630904
1552266_at 3.200243154

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM596054.CEL.gz 4.5 Mb (ftp)(http) CEL
GSM596054.chp.gz 393.0 Kb (ftp)(http) CHP
Processed data included within Sample table

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