|
| Status |
Public on Sep 20, 2010 |
| Title |
HMLE, shEcad, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HMLE breast epithelial cells transduced with shEcad
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HMLE
tissue: breast
genotype: SV40ER, hTERT
|
| Treatment protocol |
Following retroviral transduction, cells were treated for 5 days with blasticidin (5ng/ml); after drug selection, cells were grown for at least one week prior to RNA extraction.
|
| Growth protocol |
Cells were grown in a 1:1 mixture of DMEM+10%FBS, insulin, hydrocortisone and MEGM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from culture plates for each cell type using an RNeasy Mini kit coupled with an RNase-free DNase set (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol; hybridization was performed with high-throughput HT_HG-U133A arrays in 96-well format
|
| Scan protocol |
Scanned using an Affymetrix HT Scanner
|
| Description |
Global gene expression signature upon salinomycin treatment
|
| Data processing |
The data were RMA-normalized using the open-source R-project statistical software with bioconductor packages.
|
| |
|
| Submission date |
Sep 17, 2010 |
| Last update date |
Sep 20, 2010 |
| Contact name |
Piyush B. Gupta |
| E-mail(s) |
[email protected]
|
| Phone |
617-324-0086
|
| Organization name |
Whitehead Institute for Biomedical Research/MIT
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL3921 |
| Series (1) |
| GSE24202 |
Expression data from genetically modified HMLE human mammary epithelial cells |
|
