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GEO help: Mouse over screen elements for information. |
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Status |
Public on Sep 20, 2010 |
Title |
Expression data from genetically modified HMLE human mammary epithelial cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Microarrays were used to determine relative global gene expression changes upon introduction of EMT-inducing or control vectors.
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Overall design |
Experimentally immortalized HMLE breast epithelial cells were retrovirally transduced in culture with vectors encoding EMT-inducing genes or control vectors. There were three biologic replicates for each genetic perturbation condition. After treatment, the cells were selected and expanded, after which total RNA was isolated and subjected to microarray analysis.
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Contributor(s) |
Gupta PB, Mani SA, Onder TT, Gupta S, Lander ES, Weinberg RA |
Citation(s) |
20713713 |
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Submission date |
Sep 17, 2010 |
Last update date |
Jan 17, 2017 |
Contact name |
Piyush B. Gupta |
E-mail(s) |
[email protected]
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Phone |
617-324-0086
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Organization name |
Whitehead Institute for Biomedical Research/MIT
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Street address |
9 Cambridge Center
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City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
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Platforms (1) |
GPL3921 |
[HT_HG-U133A] Affymetrix HT Human Genome U133A Array |
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Samples (21)
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GSM595329 |
HMLE, TGFb, rep1 |
GSM595330 |
HMLE, TGFb, rep2 |
GSM595331 |
HMLE, TGFb, rep3 |
GSM595332 |
HMLE, Twist, rep1 |
GSM595333 |
HMLE, Twist, rep2 |
GSM595334 |
HMLE, Twist, rep3 |
GSM595335 |
HMLE, Gsc, rep1 |
GSM595336 |
HMLE, Gsc, rep2 |
GSM595337 |
HMLE, Gsc, rep3 |
GSM595338 |
HMLE, Snail, rep1 |
GSM595339 |
HMLE, Snail, rep2 |
GSM595340 |
HMLE, Snail, rep3 |
GSM595341 |
HMLE, shGFP, rep1 |
GSM595342 |
HMLE, shGFP rep2 |
GSM595343 |
HMLE, shGFP rep3 |
GSM595344 |
HMLE, shEcad, rep1 |
GSM595345 |
HMLE, shEcad, rep2 |
GSM595346 |
HMLE, shEcad, rep3 |
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Relations |
BioProject |
PRJNA130177 |
Supplementary file |
Size |
Download |
File type/resource |
GSE24202_RAW.tar |
56.0 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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