|
| Status |
Public on Aug 21, 2010 |
| Title |
PaS_4factor_5 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow PaS (MSC), infected Oct3/4, Sox2, Klf4, and c-Myc
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Bone marrow
cell source: PaS (MSC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified with Trizol (invitrogen) and treated with Turbo DNA-free kit (Ambion) to remove genomic DNA contamination.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from ES cells, PαS cells, OB cells, TTF cells and iPS cells were labelled with Cy3.
|
| |
|
| Hybridization protocol |
Samples were hybridized to a mouse Oligo Microarray (agilent) according to the manufacturer's protocol.
|
| Scan protocol |
Arrays were scanned with a G2565BA Microarray Scanner System (Agilent).
|
| Data processing |
Data were analysed using GeneSprings GX software (Agilent).
|
| |
|
| Submission date |
Aug 19, 2010 |
| Last update date |
Aug 20, 2010 |
| Contact name |
Yumi Matsuzaki |
| E-mail(s) |
[email protected]
|
| Phone |
81-3-5363-3117
|
| Fax |
81-3-5363-3566
|
| Organization name |
Keio University, School of Medicine
|
| Department |
Physiology
|
| Street address |
35 Shinanomachi
|
| City |
Shinjuku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE23717 |
Purified mesenchymal stem cells are an efficient source for iPS cell induction |
|
