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Sample GSM5821094 Query DataSets for GSM5821094
Status Public on Jun 30, 2023
Title WT1
Sample type SRA
 
Source name Bacterial cells
Organism Xanthomonas citri pv. glycines
Characteristics strain: Strain 12-2
genotype: wild-type
Extracted molecule total RNA
Extraction protocol Total RNA from each strain was isolated using an RNeasy® Mini Kit (QIAGEN) and contaminating DNA was removed using TURBO DNA-free™ Kit (Ambion, Austin, TX, U.S.A.).
Libraries were prepared using Ribo-Zero rRNA Removal Kit (Bacteria), TruSeq RNA Sample Prep Kit v2 following the Illumina manufacturer’s instructions
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing The Illumina sequencer generates raw images utilizing sequencing control software for system control and base calling through an integrated primary analysis software called RTA (Real Time Analysis).
The BCL (base calls) binary is converted into FASTQ utilizing illumina package bcl2fastq.
Sequenced reads were trimmed for adaptor sequence, and then mapped to Xanthomonas citri pv.glycines 12-2 whole genome using Bioconductor Rsubread package with parameter align(index=" Xag12-2index ",readfile1="XagWT2_1.fastq.gz", readfile2="XagWT2_2.fastq.gz",output_file="WT2alignResults.BAM")
Genome_build: Xag12-2index
Supplementary_files_format_and_content: tab-delimited text files include read counts for each sample ...
 
Submission date Jan 17, 2022
Last update date Jun 30, 2023
Contact name Tiyakhon Chatnaparat
E-mail(s) [email protected]
Organization name Kasetsart University
Department Plant Pathology
Lab Phytobacteriology
Street address 50 Ngamwongwan Rd. Ladyao, Chatuchak
City Bangkok
ZIP/Postal code 10900
Country Thailand
 
Platform ID GPL31233
Series (1)
GSE193787 Global gene expression profiles of phoB and phoU mutants of X. citri pv. glycines 12-2 conducted under phosphate limitation conditions in vitro using RNA-seq
Relations
BioSample SAMN25042800
SRA SRX13811073

Supplementary file Size Download File type/resource
GSM5821094_WT1countsnew.txt.gz 31.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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