|
| Status |
Public on Apr 15, 2022 |
| Title |
He_Cds_5W-8 |
| Sample type |
RNA |
| |
|
| Source name |
heart, Candesartan 5 milligram per kilogram per day
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: heart
Sex: male
age: 16 weeks
strain: WKY/Izm
individual: W310-8
array: 252828210261
scan date: 2011.02.16
compound: Candesartan
dose: 5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were obtained from male rats at 16 weeks old. The RNA was extracted using RNeasy kit (Qiagen) or AllPrep DNA/RNA Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
The RNA samples were labeled using the Low Input Quick Amp Labeling Kit (Agilent). cRNA samples were prepared with a template of 100 ng of total RNA and labeled with Cy3.
|
| |
|
| Hybridization protocol |
RNA samples were prepared and analyzed using the Rat GE 4x44K v3 Microarray (Agilent Microarray Design ID 028282).
|
| Scan protocol |
Arrays were scanned using the G2539A scanner (Agilent) until 2011.07.01 and the G2600D scanner (Agilent) from 2014.06.19.
|
| Data processing |
The scanned images were processed using the Feature Extraction software (Agilent). The signal intesity was transformed by the logarithm of base 2.
|
| |
|
| Submission date |
Nov 06, 2021 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Fumihiko Takeuchi |
| E-mail(s) |
[email protected]
|
| Organization name |
National Center for Global Health and Medicine
|
| Department |
Research Institute
|
| Street address |
1-21-1 Toyama
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14746 |
| Series (2) |
| GSE188348 |
Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration [heart] |
| GSE188350 |
Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration |
|
