|
| Status |
Public on May 07, 2023 |
| Title |
D542G_ML_E2/P_sample2 |
| Sample type |
SRA |
| |
|
| Source name |
Mammary mature luminal cells
|
| Organism |
Mus musculus |
| Characteristics |
er mutation: D542G
treatment: E2+P4
cell type: Mammary mature luminal cells
|
| Treatment protocol |
Animals were ovariectomized, then implanted with hormone pellets (placebo, E2, or P4). Hormone pellet incubation for 8 days post-implantation, then tissue harvest.
|
| Growth protocol |
Animals were raised under standard husbandry methods until 8 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mammary glands were dissected from treated animals; mature luminal cells were isolated from tissues by FACS. RNA was isolated from mature luminal cells via Qiagen Rneasy Micro Plus kit.
Libraries were prepared from cDNA using the Nextera XT DNA Sample Preparation kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
SAM24376721
|
| Data processing |
HTSeqGenie pipeline
Genome_build: GRCm38
Supplementary_files_format_and_content: TAB (Raw counts and rpkm per gene)
|
| |
|
| Submission date |
Oct 23, 2021 |
| Last update date |
May 08, 2023 |
| Contact name |
Jackson Liang |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech
|
| Department |
Discovery Oncology
|
| Lab |
Metcalfe Lab
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE186453 |
Evaluating hormone responses in Esr1-wildtype and mutant mammary luminal cells [RNA-seq] |
|
| Relations |
| BioSample |
SAMN22545903 |
| SRA |
SRX12750916 |
