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| Status |
Public on May 07, 2023 |
| Title |
Evaluating hormone responses in Esr1-wildtype and mutant mammary luminal cells [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To examine the effects of hormone stimulation on Esr1-wildtype and Esr1-mutant mammary glands, we will ovariectomize both wildtype and ER-mutant mice. Following ovariectomy (OVX), animals will be implanted with hormone pellets (either placebo, estrogen, and/or progesterone). Then, we perform RNA-seq on FACS-sorted mature luminal cells (ML) from mammary glands of treated mice. This analysis will allow for separation of ER mutant-intrinsic effects on gene expression vs. combinatorial effects from exposure to either estrogen or progesterone.
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| |
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| Overall design |
37 samples are included, 3 biological replicates per condition (except for the 'WT + P4' condition, which has 4 biological replicates). 'WT + placebo' condition is used as a reference.
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/36130016/
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| |
|
| Contributor(s) |
Liang J, Hafner M, Metcalfe C |
| Citation(s) |
36130016 |
| |
| Submission date |
Oct 23, 2021 |
| Last update date |
May 10, 2023 |
| Contact name |
Jackson Liang |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech
|
| Department |
Discovery Oncology
|
| Lab |
Metcalfe Lab
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (37)
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|
| Relations |
| BioProject |
PRJNA773928 |
| SRA |
SRP342831 |
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