|
| Status |
Public on Dec 31, 2010 |
| Title |
NCI-N87 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
NCI-N87
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: gastric cancer cell line NCI-N87
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extacted using Qiagen DNA extraction kit.
|
| Label |
Cy3-dUTP
|
| Label protocol |
Gastric genomic DNA were hybed with Cy3-dUTP, and the control human spleen DNA were labeled with Cy5-dUTP.
|
| |
|
| Channel 2 |
| Source name |
control human spleen genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: spleen
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extacted using Qiagen DNA extraction kit.
|
| Label |
Cy5-dUTP
|
| Label protocol |
Gastric genomic DNA were hybed with Cy3-dUTP, and the control human spleen DNA were labeled with Cy5-dUTP.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added,
|
| Scan protocol |
Hybridized slides were scanned on an Agilent DNA Microarray Scanner
|
| Description |
NA
|
| Data processing |
Images were extracted using Agilent Feature Extraction software(9.1.3). Data was analyzed using Agilent CGH Analytics software (v.3.5)
|
| |
|
| Submission date |
Jul 05, 2010 |
| Last update date |
Nov 15, 2012 |
| Contact name |
Chia-Huey Ooi |
| Organization name |
F. Hoffmann-La Roche Ltd.
|
| Street address |
Grenzacherstrasse 124
|
| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL4544 |
| Series (1) |
| GSE22694 |
Copy number profiling of gastric cancer cell lines and gastric tumor tissues |
|
