|
| Status |
Public on Aug 26, 2021 |
| Title |
Contusion injury cord, 28DPI, biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Contusion injury cord, 28DPI
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
Sex: Female
age: Adult
tissue: Spinal cord tissue
injury: 175 kDyn, T10 contusion
treatment: None
time post-injury: 28 days
|
| Treatment protocol |
Adult female Sprague-Dawley rats (220-250 g) underwent surgical T10 laminectomy and moderate contusive injury (175 kDyn) by an Infinite Horizons impactor. Treatment groups were acutely administered the AAV5-GfaABC1D-dYFP reporter vector at the time of surgery by stereotaxic microinjection. Following recovery, at 7- and 28-days post-injury animals were euthanised, perfused with ice-cold saline and a 5 mm region centered on the spinal lesion excised to be snap frozen.
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by mechanical tissue dissociation, TRIzol / chloroform extraction and phase separation. RNA extract quantified by Implen NP80 nanophotometer and quality assed by Agilent Bioanalyzer (RIN >8.80).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
|
| |
|
| Hybridization protocol |
Rat Clariom S arrays were incubated in the Affymetrix® GeneChip® Hybridization Oven 645 at 45° C / 60 RPM for 16-18 hours.
|
| Scan protocol |
GeneChips were scanned with the GeneChip™ Scanner 3000 (Applied Biosystems, #00-0186) according to manufacturer's instructions.
|
| Description |
SD_Rat_Injury_28DPI_05
Rattus norvegicus domestica
|
| Data processing |
Raw intensity CEL files were imported into Transcriptome Analysis Console (TAC software v4.0.2.15. Applied Biosystems), and gene level expression analysis was performed using the default ANOVA settings.
|
| |
|
| Submission date |
Aug 25, 2021 |
| Last update date |
Aug 26, 2021 |
| Contact name |
Connor Andrew Clemett |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Auckland
|
| Department |
Anatomy and Medical Imaging
|
| Street address |
85 Park Rd
|
| City |
Auckland |
| State/province |
Auckland |
| ZIP/Postal code |
1025 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL23040 |
| Series (1) |
| GSE182796 |
Astrocyte-selective transduction with AAV5 viral vector does not exacerbate astrogliosis or alter tissue immune responses following spinal injury |
|
