|
| Status |
Public on Jun 16, 2022 |
| Title |
EIPA-treated GM03813 fibroblasts, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
GM03813
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 10 microM EIPA
|
| Treatment protocol |
GM03813 cells were treated with either 10 microM amiloride, 10 microM EIPA or and equivalent volume of DMSO for 5 days at 37C.
|
| Growth protocol |
GM03813 type II SMA fibroblasts were grown in DMEM supplemented with 1% glutamine, pen/strept and 10% EqualFetal serum 24 hours prior to treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using the Rnease Mini kit (QIAGEN) according to manufacturer's directions.
|
| Label |
biotin
|
| Label protocol |
Fragmented cDNA was end-labeled with a biotinylated dideoxynucleotide using terminal transferase.
|
| |
|
| Hybridization protocol |
The probes were fragmented and then hybridized for 16 hours at 45C on Clariom D Human GeneChip Arrays in a GeneChip Hybridization Oven 645. The arrays were washed and stained in the GeneChip Fluidic Station 450.
|
| Scan protocol |
The arrays were scanned with the GeneChip 3000 scanner.
|
| Description |
gene expression data from treated SMA fibroblasts
|
| Data processing |
Raw signal intensities were analyzed with TAC 4.0 software (Applied Biosystems)
|
| |
|
| Submission date |
Jul 10, 2021 |
| Last update date |
Jun 16, 2022 |
| Contact name |
Matthew E R Butchbach |
| E-mail(s) |
[email protected]
|
| Organization name |
Nemours Children's Hospital Delaware
|
| Department |
Neurology
|
| Lab |
Motor Neuron Diseases Research Laboratory
|
| Street address |
200 Powder Mill Road, 4462 E400
|
| City |
Wilmington |
| State/province |
DE |
| ZIP/Postal code |
19803 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE179861 |
Expression data from SMA fibroblasts treated with amiloride or EIPA |
|
