|
| Status |
Public on Oct 29, 2021 |
| Title |
Spt3_rep_2 |
| Sample type |
SRA |
| |
|
| Source name |
Ovary
|
| Organism |
Drosophila melanogaster |
| Characteristics |
chip antibody: Spt3
genotype: wild type
|
| Treatment protocol |
N/A
|
| Growth protocol |
Kc167 cells were maintained at 1-2 e6 cells/mL in SFX medium (HyQ SFX-Insect;HyClone Laboratories, Inc) at 27 dC. Drosophila melanogaster were grown on standardsyrup medium at 25 °C.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
ChIP-seq
|
| Data processing |
bcl2fastq2-v2.20.0.422 was used to generate fastq files.
Reads were aligned to dm6 genome using bowtie2-2.3.4.1 with the default parameters.
Genome_build: dm6
|
| |
|
| Submission date |
Feb 04, 2021 |
| Last update date |
Oct 29, 2021 |
| Contact name |
Hua Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Stowers Institute for Medical Research
|
| Street address |
1000 E 50th Street
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE166224 |
SAGA core module subunits regulate gene expression during oogenesis [ChIP-seq] |
|
| Relations |
| BioSample |
SAMN17800164 |
| SRA |
SRX10031707 |
