|
| Status |
Public on May 01, 2021 |
| Title |
SC with stiff ECM |
| Sample type |
RNA |
| |
|
| Source name |
High ECM stiffness
|
| Organism |
Rattus norvegicus |
| Characteristics |
passage number: 5
|
| Treatment protocol |
PDMS cell culture substrates were coated with laminin to increase cell adhesion. PDMS and micropatterned cell culture substrates were sterilized with 70% ethanol for 30 minutes before seeding cells.
|
| Growth protocol |
SCs were cultured under standard cell culture condition (37°C, 5% CO2) for 2 days before RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen) was used to extract total RNA according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
GeneChip™ WT PLUS Reagent Kit (902280, Thermo Fisher) was used to generate biotin labeled single-stranded complementary DNA
|
| |
|
| Hybridization protocol |
2.3 µg of fragmented cDNA was hybridized to Rat Clariom S Arrays (902934, Thermo Fisher) by incubating at 45°C for 17 hours before washing and staining with the GeneChip™ Hybridization, Wash, and Stain kit (900720, Thermo Fisher).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data for SCs with stiff ECM
|
| Data processing |
The raw data files were generated by Command Console (Affymetrix). The data were analyzed using the Transcriptome Analysis Console (TAC) Software in combination with Rstudio.
|
| |
|
| Submission date |
Jan 20, 2021 |
| Last update date |
May 02, 2021 |
| Contact name |
zhenyuan xu |
| E-mail(s) |
[email protected]
|
| Phone |
5133020366
|
| Organization name |
University of Cincinnati
|
| Department |
Chemical Engineering
|
| Street address |
2378 Montana Ave, AptE
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45211 |
| Country |
USA |
| |
|
| Platform ID |
GPL23040 |
| Series (1) |
| GSE165206 |
Expression data from Rat neuronal Schwann cells (SCs) that under different extracellular matrix (ECM) conditions |
|
