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| Status |
Public on May 25, 2021 |
| Title |
patient 1 CD84 activation sorted G-MDSCs |
| Sample type |
SRA |
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| Source name |
sorted G-MDSCs
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| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
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| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were seperated by ficoll then sorted for G-MDSCs, thereafter seperated into two samples and treated with anti-CD84 activating antibody or IgG
Libraries were prepared with the MARS-seq protocol (Diego Adhemar Jaitin, 2017) and sequenced using Illumina NextSeq 500 High Output v2 Kit (75 cycles).
MARS-seq
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
none provided by the submitter
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| Submission date |
Jan 12, 2021 |
| Last update date |
May 25, 2021 |
| Contact name |
Idit Shachar |
| E-mail(s) |
[email protected]
|
| Organization name |
Weizmann Institute of science
|
| Department |
Immunology
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| Lab |
Prof. Idit Shachar
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| Street address |
Herzl 234
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| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platform ID |
GPL18573 |
| Series (1) |
| GSE164651 |
Next Generation Sequencing Facilitates Quantitative Analysis of human multiple myeloma derived CD84 activated G-MDSCs or IgG (control) treated G-MDSCs |
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| Relations |
| BioSample |
SAMN17293883 |
| SRA |
SRX9832942 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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