|
| Status |
Public on Jan 19, 2021 |
| Title |
CD45lung_NS-RA_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
control pulmonary immune cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
tissue: lung
cell type: pulmonary immune cells
age: 2-week old
treatment: Prenatal normal saline and postnatal normoxia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were directly sorted into aliquots of 500ul of RLT lysis buffer (QIAgen), homogenized in a QIA-shredder (QIAgen), and stored in -80C. Total RNA was extracted using Qiagen All prep kit.
|
| Label |
Biotin
|
| Label protocol |
cDNA was label with biotin by using WT-Plus kit.
|
| |
|
| Hybridization protocol |
Mouse Clariom S gene chips were hybridize with 2.5 μg fragmented and biotin-labeled cDNA in 100 μl of hybridization cocktail.
|
| Scan protocol |
Chips were scanned on an Affymetrix Gene Chip Scanner 3000 7G, using Command Console Software.
|
| Description |
Prenatal normal saline and postnatal normoxia
|
| Data processing |
Exp. group was compare with cont. group by using Transcriptome analysis console (TAC 4.0) software.
Rat clariom S
Expression values (Log2 value) of all gene list analyzed by TAC 4.0 software
|
| |
|
| Submission date |
Nov 19, 2020 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Sankar Addya |
| E-mail(s) |
[email protected]
|
| Organization name |
Thomas Jefferson University Hospital
|
| Department |
Kimmel Cancer Center
|
| Lab |
Cancer Genomics Lab
|
| Street address |
233 South 10th Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL23040 |
| Series (1) |
| GSE161836 |
Pulmonary immune cell transcriptome changes in double-hit model of BPD induced by chorioamnionitis and postnatal hyperoxia |
|
