|
| Status |
Public on Jul 01, 2021 |
| Title |
CD90+EPCR- HSC 6 [AFO296A15] |
| Sample type |
SRA |
| |
|
| Source name |
CD90+EPCR- HSC
|
| Organism |
Homo sapiens |
| Characteristics |
age: post-natal
tissue: cord blood
cell type: progenitor (CD34+CD38-CD45RA-CD90+EPCR-) cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA using RNeasy Plus Micro Kit (Qiagen) and quality was verified in an Agilent 2100 Bioanalyzer (Agilent).
KAPA Stranded with RiboErase RNA-seq kit (Kapa Biosystems)
RNA-seq 30 million single-end 100 bp reads/sample
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
AFO296A15_S16
AFO296A15_S25
|
| Data processing |
Raw sequencing reads were mapped with STAR
Expression counts estimates and transcripts per million (TPM) were generated using RSEM
Genome_build: GRCh38
Supplementary_files_format_and_content: raw counts
|
| |
|
| Submission date |
Jul 12, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE154263 |
Newly defined Human Hematopoietic Stem Cell Populations |
|
| Relations |
| BioSample |
SAMN15512860 |
| SRA |
SRX8713869 |
