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| Status |
Public on Jul 01, 2021 |
| Title |
Newly defined Human Hematopoietic Stem Cell Populations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: to compare the transcriptomic profile of the two most primitive human EPCR+ stem and CD90+EPCR- progenitor populations.
Methods: cells were obtained from human cord-blood and flow-sorted using the surface antigens described. 5 EPCR+ and 4 CD90+EPCR- biological replicates were used to extract total RNA and quality was verified (RIN >8).
Libraries were prepared using with 20-25 ng/sample of starting RNA. All samples were sequenced two independent times.
Conclusions: this study represents the first analysis of transcriptomes from the two most primitive human stem/progenitor populations, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles of other human blood stem/progenitor populations.
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| Overall design |
Transcriptomic profiling (RNA-seq) of the two most primitive human stem (CD34+CD38-CD45RA-EPCR+; hereafter EPCR+) and progenitor (CD34+CD38-CD45RA-CD90+EPCR-; hereafter CD90+EPCR-) populations.
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| Contributor(s) |
Anjos-Afonso F, Buettner F, Bonnet D |
| Citation(s) |
35440586 |
| |
| Submission date |
Jul 12, 2020 |
| Last update date |
Jan 26, 2024 |
| Contact name |
Florian Buettner |
| Organization name |
DKFZ
|
| Department |
DKTK Frankfurt
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt |
| State/province |
Germany |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA645689 |
| SRA |
SRP271450 |
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